|
|
(78 intermediate revisions not shown) |
Line 1: |
Line 1: |
| {{Michigan Header}} | | {{Michigan Header}} |
| | | |
- | {|style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="62%"
| + | =Pili Expression Team= |
- | |-
| + | |
- | !
| + | |
- | |Sunday
| + | |
- | |Monday
| + | |
- | |Tuesday
| + | |
- | |Wednesday
| + | |
- | |Thursday
| + | |
- | |Friday
| + | |
- | |Saturday
| + | |
- | |-
| + | |
- | !Week 1
| + | |
- | | -
| + | |
- | |[[Team:Michigan/Pili_Expression#6/28/2010|6/28/2010]]
| + | |
- | |[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]]
| + | |
- | |[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]]
| + | |
- | |[[Team:Michigan/Pili_Expression#7/1/2010|7/1/2010]]
| + | |
- | | -
| + | |
- | | -
| + | |
- | |-
| + | |
- | !Week 2
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | [[Team:Michigan/Pili_Expression#7/7/2010|7/7/2010]]
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | |-
| + | |
- | !Week 3
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | |-
| + | |
- | !Week 4
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | | -
| + | |
- | |}
| + | |
| | | |
- | =Pili Expression Team=
| |
| This team includes [[User:MarcSinger|Marc Singer]], [[User:kevijose|Kevin Joseph]], and [[User:shanwu|Alena Wu]]. | | This team includes [[User:MarcSinger|Marc Singer]], [[User:kevijose|Kevin Joseph]], and [[User:shanwu|Alena Wu]]. |
| | | |
- | ==6/28/2010== | + | ==Monthly Notebooks== |
- | Made a 500 mL batch of LB broth
| + | In order to conserve page space, we have split our notebook according to months. |
- | *When pouring in distilled water, pour a few mL at a time to avoid clumping.
| + | |
- | *It takes 20g of powder to make 1 L of broth
| + | |
| | | |
- | Sterilized broth using the autoclave
| + | [[Team:Michigan/Pili_June_July|June/July]] |
- | *The temperature setting on the autoclave is off by a little bit
| + | |
- | *Set dial 2 notches below 134°C.
| + | |
| | | |
- | ==6/29/2010==
| + | [[Team:Michigan/Pili_August_September|August/September]] |
- | Started growing E. coli K12 cultures
| + | |
- | *Poured 2 mL of LB broth into a Falcon tube
| + | |
- | *Used strain of K12 from Dr. Lin's freezer
| + | |
- | *Placed in incubator shaker for 24 hrs.
| + | |
| | | |
- | Added and inventoried supplies from Dr. Pinto's lab.
| + | [[Team:Michigan/Pili_October|October]] |
- | *Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
| + | |
- | *Chemical wastes must be cleaned by calling OSEH.
| + | |
| | | |
- | ==6/30/2010== | + | =='''In the Lab'''== |
- | Cryopreserved stock of K12
| + | <html> |
- | *1 mL located in the iGEM box in the Lin lab.
| + | <iframe src="http://www-personal.umich.edu/~shanwu/wiki_photos/src/themes/classic/classic-demo_pili.html" width="100%" height="650px" scrolling="no"></iframe> |
- | *1 mL located in the lab freezer.
| + | </html> |
- | | + | |
- | ==7/1/2010==
| + | |
- | Cryopreserved DH5α according to protocol procedure on 6/30/2010
| + | |
- | *Put 1 stock in -20°C fridge in 1239
| + | |
- | *Put the other stock in the -80°C fridge in the Lin lab
| + | |
- | | + | |
- | ==7/7/2010== | + | |
- | Obtain genomic DNA of CFT073 E. coli strain from Dr. Mobley's Lab
| + | |
- | *stored in -20°C fridge on ice
| + | |