Team:UNIPV-Pavia/Calendar/July
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<table border="0" align="center" width="100%" class="trasparenza"><tr><td align="justify" valign="top" style="padding:20px"> | <table border="0" align="center" width="100%" class="trasparenza"><tr><td align="justify" valign="top" style="padding:20px"> | ||
- | <html><p align="center"><font size="4" | + | |
- | + | <table class="menu" border="0" width="100%"> | |
+ | <tr> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/July/settimana1|Week 1]] | ||
+ | </td> | ||
+ | <td align="center" style="padding:0; height:20px"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/July/settimana2|Week 2]] | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/July/settimana3|Week 3]] | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/July/settimana4|Week 4]] | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/July/settimana5|Week 5]] | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | <html><p align="center"><font size="4"><b>JULY</b></font></p></html><hr><br> | ||
+ | Bio-Lab activity proceeds!<br><br> | ||
+ | <font size = "4"><b><u>Select the day you are interested in to find out the details!</u></b></font><br><br> | ||
<table class=cont border="0" width="30%" align="center" valign="top"> | <table class=cont border="0" width="30%" align="center" valign="top"> | ||
<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | ||
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 1st|July, 1st]]</td><td align="left">19° Bio-Lab - | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 1st|July, 1st]]</td><td align="left"> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 2nd|July, 2nd]]</td><td align="left">20° Bio-Lab - | + | 19° Bio-Lab - I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test.<br> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left"> | + | Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 6th|July, 6th]]</td><td align="left"> | + | Other parts were received from Anderson Lab. |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 7th|July, 7th]]</td><td align="left"> | + | </td></tr> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 8th|July, 8th]]</td><td align="left"> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 2nd|July, 2nd]]</td><td align="left"> |
+ | 20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP.<br> | ||
+ | Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . | ||
+ | Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture.<br> | ||
+ | Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 3rd|July, 3rd]]</td><td align="left"> | ||
+ | 21° Bio-Lab - Glycerol stock for <partinfo>BBa_J72008</partinfo> was prepared. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 2</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left"> | ||
+ | 22° Bio-Lab - LB agar plates prepared. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 6th|July, 6th]]</td><td align="left"> | ||
+ | 23° Bio-Lab - We decided to perform a TECAN test to evaluate the strength of some promoters belonging to the Anderson Promoters Collection. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 7th|July, 7th]]</td><td align="left"> | ||
+ | 24° Bio-Lab -Competent cells preparation for: | ||
+ | * MG1655 | ||
+ | * BW53474 | ||
+ | * BW25141 | ||
+ | * BW25142 | ||
+ | PCR was performed to amplify Phasins. | ||
+ | <br> | ||
+ | 11° Meeting - Updating about Bio-Lab activity. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 8th|July, 8th]]</td><td align="left"> | ||
+ | 25° Bio-Lab - Transformation of <partinfo>BBa_J23118</partinfo> in our home-made competent strains | ||
* MG1655 | * MG1655 | ||
* BW25141 | * BW25141 | ||
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* BW53474 | * BW53474 | ||
*DH5alpha (as control) | *DH5alpha (as control) | ||
- | to check transformation efficiency.</td></tr> | + | to check transformation efficiency. |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 9th|July, 9th]]</td><td align="left"> | + | </td></tr> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 12th|July, 12th]]</td><td align="left"> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 9th|July, 9th]]</td><td align="left"> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 13th|July, 13th]]</td><td align="left"> | + | 26° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains. |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 14th|July, 14th]]</td><td align="left"> | + | </td></tr> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 15th|July, 15th]]</td><td align="left"> | + | <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 3</b></th></tr> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 16th|July, 16th]]</td><td align="left"> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 12th|July, 12th]]</td><td align="left"> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 19th|July, 19th]]</td><td align="left"> | + | 27° Bio-Lab - Phasins PhaP1 and PhaP2 were amplified by PCR and the results were sequenced . |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 20th|July, 20th]]</td><td align="left"> | + | </td></tr> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 13th|July, 13th]]</td><td align="left"> |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, | + | 28° Bio-Lab - Phasins samples were sent to be sequenced. MiniPrep was performed. Ligations (from I11 till I19 and I74c5, I84c5, I104c5) were all performed |
- | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, | + | </td></tr> |
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 14th|July, 14th]]</td><td align="left"> | ||
+ | 29° Bio-Lab - PBHR68 plate showed colonies!!transformation of ligation of yesterday. Screening of <partinfo>BBa_J72007</partinfo> (CRIM), <partinfo>BBa_J72008</partinfo> (helper), pAH123 (helper) ans pCP20 (helper). | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 15th|July, 15th]]</td><td align="left"> | ||
+ | 30° Bio-Lab -Agar plates after transformation were checked(2 colonies were picked from every plate). | ||
+ | MiniPrep was performed for: | ||
+ | * PBHR68 (BioPlastic operon) -> 272 ng/ul | ||
+ | * BBa_J72007(CRIM plasmid) -> 41 ng/ul (not clean spectrum at 230nm) | ||
+ | * BBa_J72013(CRIM plasmid) -> 16 ng/ul | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 16th|July, 16th]]</td><td align="left"> | ||
+ | 31° Bio-Lab - MiniPrep was performed on 23 falcon tubes containing I11 -> I19 and I7/8/10-4C5 ligations. | ||
+ | We decided to performe a TECAN test on our parts, in order to see if RFP was correctly excided from BBa_J231xx vector, since length of RFP and of our insert (RBS-luxI-tt) were similar. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 4</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 19th|July, 19th]]</td><td align="left"> | ||
+ | 32° Bio-Lab - TECAN test showed that no RFP was produced from our parts.I14-1, I16-1, I17-1, I18-1, I19-1, I74c5-2, I84c5-2 and I12-2 were sequenced. | ||
+ | Trasformation of RING into: | ||
+ | * BW25141 (pir+) | ||
+ | * BW25142 (pir116) | ||
+ | * BW23474 (pir116) | ||
+ | * DH5alpha | ||
+ | * MG1655 | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 20th|July, 20th]]</td><td align="left"> | ||
+ | 33° Bio-Lab - Agar plates after transformation were checked (Single colonies were picked from plates). | ||
+ | MiniPrep was performed on I10-1, I12-2, I3-1, I14-1, I17-1 and <partinfo>BBa_J23110</partinfo>. | ||
+ | Following ligations were performed: I15_new, I14_4C5, I16_4C5, I17_4C5, I18_4C5 and I19_4C5. | ||
+ | We incoulated from glycerol stock (3ul in 2ml LB+Amp): | ||
+ | <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_J23118</partinfo>, <partinfo>BBa_J23116</partinfo>, <partinfo>BBa_J23114</partinfo>, <partinfo>BBa_J23106</partinfo>, <partinfo>BBa_J23105</partinfo>, <partinfo>BBa_J23101</partinfo>, <partinfo>BBa_J23100</partinfo>, <partinfo>BBa_B0033</partinfo> | ||
+ | in order to perform a TECAN test tomorrow. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 21st|July, 21st]]</td><td align="left"> | ||
+ | 34° Bio-Lab -Tecan Test. This morning all the seven cultures were grown, For those cultures glycerol stocks were prepared and stored at -80°C. (Remaning 5 ml were used to perform MiniPrep). | ||
+ | Competent cells for cultures inoculated yesterday were prepared. Trasformations of ligations of yesterday were performed. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 22nd|July, 22nd]]</td><td align="left"> | ||
+ | 35° Bio-Lab - Plates were checked. | ||
+ | Following ligations were performed: I17_4C5, I18_4C5, I19_4C5, I10-4C5, I12-4C5. | ||
+ | Competent T9002 and MC1061 cells were transformed with a negative control in pSB4C5. | ||
+ | We also performed a TECAN test in order to evaluate the ranking of sternght of promoters we inoculaed yesterday. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 23rd|July, 23rd]]</td><td align="left"> | ||
+ | 36° Bio-Lab - All plates incubated yesterday (T9002-4C5, MC1051-4C5 and PBHR68-4C5) showed colonies! | ||
+ | Trasformations of ligations of yesterday were performed. | ||
+ | We also received sequencing results (all correct). | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 24th|July, 24th]]</td><td align="left"> | ||
+ | 37° Bio-Lab - Two colonies from each plate incubated yesterday were picked. | ||
+ | Colonies were counted to evaluate efficiency of transformation for our home made competent cells. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana4 #July, 25th|July, 25th]]</td><td align="left"> | ||
+ | 38° Bio-Lab - Screening digestion was performed for colonies picked yesterday. MiniPrep and gel run was performed. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 5</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 26th|July, 26th]]</td><td align="left"> | ||
+ | 39° Bio-Lab - Ligation of I15_4C5. <br> | ||
+ | 12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston! | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 27th|July, 27th]]</td><td align="left"> | ||
+ | 40° Bio-Lab - Sample of I15-1 was prepared for sequencing.I14_4C5, I16_4C5, I17_4C5, I18_4C5 and I19_4C5 were cotrasformed in T9002. | ||
+ | MiniPrep was performed on MC1, MC2. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 28th|July, 28th]]</td><td align="left"> | ||
+ | 41° Bio-Lab - Today we diluted primers to modify PhaPs and performed a PCR with them in order to mutagenize this BioBrick to get its prefix Standard/Silver compliant and suffix Silver compliant. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 29th|July, 29th]]</td><td align="left"> | ||
+ | 42° Bio-Lab - PCR was prepared in order to amplify Phasins Phap (<partinfo>BBa_K208001</partinfo>).After that phasins were gel extracted and digested. | ||
+ | A tecan test was performed. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 30th|July, 30th]]</td><td align="left"> | ||
+ | 43° Bio-Lab - Agar plates were checked and efficiency was calculated. | ||
+ | Miniprep of <partinfo>BBa_J04450</partinfo> was performed. | ||
+ | Ligation of I20, I21. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #July, 31th|July, 31th]]</td><td align="left"> | ||
+ | 44° Bio-Lab - Transformation of I20 and I21 into E. coli DH5-alpha. | ||
+ | </td></tr> | ||
</table> | </table> | ||
</td> | </td> |
Latest revision as of 12:49, 22 October 2010
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