Team:Michigan/Jeremy's Notebook
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Composition of Medium | Composition of Medium | ||
- | + | *100 mg Naphthenic acids /L | |
(Modified Bushnell-Has medium) | (Modified Bushnell-Has medium) | ||
- | + | *1000 mg KH2PO4 /L | |
- | + | *1000 mg Na2HPO4 /L | |
- | + | *500 mg NH4NO3 /L | |
- | + | *500 mg (NH4)2SO4 /L | |
- | + | *200 mg MgSO4 ∙ 7H2O /L | |
- | + | *20 mg CaCl2 ∙ 2H2O /L | |
- | + | *2 mg FeCl3 /L | |
- | + | *2 mg MnSO4 ∙ 2H2O /L | |
pH of Bushnell-Has Medium: 7.0 | pH of Bushnell-Has Medium: 7.0 | ||
+ | |||
Protocol | Protocol | ||
- | + | ||
- | + | Prepare naphthenic acids stock | |
- | + | *Mix 1 g naphthenic acid /L in dilute NaOH. | |
- | + | *Adjust pH of the solution to 10-11 to dissolve naphthenic acids into the solution as sodium salts | |
- | + | Making the Growing Broth | |
- | + | *Mix 20 ml naphthenic acid stock with 170 ml sterilized water in 500 ml erlenmeyer flask. | |
- | + | *Prepare 10 ml Bushnell-Has medium in vial | |
- | + | *Mix arbitrary amount of pseudomonas strain in B-H medium | |
- | + | *Transfer medium to erlenmeyer flask | |
- | + | *Incubation (aerobic conditions) | |
+ | *Incubate stock at room temperature (20 °C) on a shaker at 200 rpm. |
Latest revision as of 04:10, 18 July 2010