Team:EPF Lausanne/Protocols

From 2010.igem.org

(Difference between revisions)
(Lab Protocols)
 
(41 intermediate revisions not shown)
Line 1: Line 1:
 +
{{EPFL_2010_2}}
 +
<html>
 +
<head> <style>
 +
 +
.EPFL_content
 +
{
 +
    margin-right:190px;
 +
}
 +
 +
</style></head>
 +
 +
<div style="position: absolute; left: 0px; top: 60px; height: 140px; width: 975px;">
 +
<img alt="" src="https://static.igem.org/mediawiki/2010/7/70/EPFL_Title_2_Protocol.png"/></div>
 +
 +
<div style="position: absolute; left: 435px; top: 11px; height: 85px; width: 125px;">
 +
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook">
 +
<img alt="" height="80" src="https://static.igem.org/mediawiki/2010/b/b9/EPFL_Menu_2_Notebook.png" width="125" class="auto-style1" /></a></div>
 +
 +
</html>
 +
 +
{{EPFL_sub_menu_notebook}}
 +
 +
 +
<div CLASS="EPFL_content">
 +
 +
<center>
 +
[[Image: asaia_protocols.png]]
 +
</center>
 +
== Lab Protocols ==
== Lab Protocols ==
-
[https://2010.igem.org/Team:EPF_Lausanne home]
 
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
-
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol]
+
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH]
-
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol]
+
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel]
-
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol]
+
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production]
-
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol]
+
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ]
-
* adapt ph (Nadine)
+
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
-
* UV imaging (Wiktor)
+
*[https://static.igem.org/mediawiki/igem.org/8/82/Prot_growing_asaia.pdf Growing Asaia]
-
* [https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
+
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
-
* [https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR protocol ]
+
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
-
* Using the plate reader (Solange)  
+
* Miniprep: use Miniprep Kit
-
* Asaia Maxiprep (effie)
+
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
-
* Asaia Miniprep (effie)
+
*[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ]
-
* Ligation (Nadine effie Stefano)
+
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof]
-
* [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells protocol ]
+
* Purification: use Purification Kit
-
* purification --> purification Kit
+
*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
 +
*[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]
 +
*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion]
 +
*[https://static.igem.org/mediawiki/2010/6/68/Prot_WesternBlot.pdf Western Blot]
Media Recipes
Media Recipes
Line 25: Line 55:
*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
 +
<b>Syntax:</b>convention if you want to upload a new protocol<br\>
 +
When uploading a file it please rename it in the following way: <i>prot_yourProcedureName.yourExtension</i>.
-
<b>Syntax: </b>convention if you want to upload a new protocol  <br\>
+
{{EPFL_2010_bottom_wrap}}
-
When uploading a file it please rename it in the following way: <i> prot_yourProcedureName.yourExtension</i>.
+
</div>

Latest revision as of 23:15, 27 October 2010



Asaia protocols.png

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Media Recipes

Syntax:convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.

wrap bottom