Team:Stanford/Notebook/Timeline/Team Analog

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(Difference between revisions)
(→Timeline for Team Analog)
(→Timeline for Team Analog)
 
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**[_] Receive primers and build promoters.
**[_] Receive primers and build promoters.
**[_] Streak BW27783 and culture to lose its current plasmid.
**[_] Streak BW27783 and culture to lose its current plasmid.
 +
**[_] Transform ligations and conduct sequencing.
**[_] Characterize parts (promoters).
**[_] Characterize parts (promoters).
-
**[_] .
+
**[_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
 +
**[_] Order more primers (degradation tags, truncated proteins, etc.).
*Week 5: 7-26-2010
*Week 5: 7-26-2010
**[_] Receive protein parts and finish with assembly.
**[_] Receive protein parts and finish with assembly.
**[_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
**[_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
 +
**[_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)
*Week 6: 8-02-2010
*Week 6: 8-02-2010

Latest revision as of 10:21, 19 July 2010

Timeline for Team Analog

  • Week 1: 6-28-2010
    • [x] Get parts and competent cells.
    • [x] Culture parts.
    • [x] Miniprep parts/make stocks.
    • [_] Characterize parts (promoters).
  • Week 2: 7-05-2010
    • [x] Restriction digest parts.
    • [x] PCR superfolded GFP reporter.
    • [x] Gel extract parts.
    • [_] Order genes for kinases, phosphatases, and transcription factors.
    • [_] Characterize parts (promoters).
  • Week 3: 7-12-2010
    • [x] Ligate parts.
    • [x] Order primers for small parts and promoters.
    • [x] Prepare MDV presentation and get money (hopefully).
    • [x] Order genes for kinases, phosphatases, and transcription factors.
    • [_] Streak BW27783 and culture to lose its current plasmid.
    • [_] Characterize parts (promoters).
  • Week 4: 7-19-2010
    • [_] Receive primers and build promoters.
    • [_] Streak BW27783 and culture to lose its current plasmid.
    • [_] Transform ligations and conduct sequencing.
    • [_] Characterize parts (promoters).
    • [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
    • [_] Order more primers (degradation tags, truncated proteins, etc.).
  • Week 5: 7-26-2010
    • [_] Receive protein parts and finish with assembly.
    • [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
    • [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)
  • Week 6: 8-02-2010
    • [_] Improve design.
  • Week 7: 8-09-2010
    • [_] Win iGEM. Already.
  • Week 8: 8-16-2010
  • Week 9: 8-23-2010
  • Week 10: 7-30-2010
    • NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon.
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