"

From 2010.igem.org

Revision as of 02:43, 17 July 2010 (view source) Axva1663 (Talk | contribs) (→Timeline for Team Analog) ← Older edit		Latest revision as of 10:21, 19 July 2010 (view source) Axva1663 (Talk | contribs) (→Timeline for Team Analog)
(2 intermediate revisions not shown)
Line 1:		Line 1:
	==Timeline for Team Analog==		==Timeline for Team Analog==
	*Week 1: 6-28-2010		*Week 1: 6-28-2010
-	**	+	**[x] Get parts and competent cells.
-	**[x] Have first meeting	+	**[x] Culture parts.
-	**[_] Get parts	+	**[x] Miniprep parts/make stocks.
-	**[_] Culture parts	+	**[_] Characterize parts (promoters).
-	**[_] Purify parts	+
-	**[_] Make stocks	+
-	**[_] Characterize parts	+
	*Week 2: 7-05-2010		*Week 2: 7-05-2010
-	**	+	**[x] Restriction digest parts.
		+	**[x] PCR superfolded GFP reporter.
		+	**[x] Gel extract parts.
		+	**[_] Order genes for kinases, phosphatases, and transcription factors.
		+	**[_] Characterize parts (promoters).
	*Week 3: 7-12-2010		*Week 3: 7-12-2010
-	**	+	**[x] Ligate parts.
		+	**[x] Order primers for small parts and promoters.
		+	**[x] Prepare MDV presentation and get money (hopefully).
		+	**[x] Order genes for kinases, phosphatases, and transcription factors.
		+	**[_] Streak BW27783 and culture to lose its current plasmid.
		+	**[_] Characterize parts (promoters).
	*Week 4: 7-19-2010		*Week 4: 7-19-2010
-	**	+	**[_] Receive primers and build promoters.
		+	**[_] Streak BW27783 and culture to lose its current plasmid.
		+	**[_] Transform ligations and conduct sequencing.
		+	**[_] Characterize parts (promoters).
		+	**[_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
		+	**[_] Order more primers (degradation tags, truncated proteins, etc.).
	*Week 5: 7-26-2010		*Week 5: 7-26-2010
-	**	+	**[_] Receive protein parts and finish with assembly.
		+	**[_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
		+	**[_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)
	*Week 6: 8-02-2010		*Week 6: 8-02-2010
-	**	+	**[_] Improve design.
	*Week 7: 8-09-2010		*Week 7: 8-09-2010
-	**	+	**[_] Win iGEM. Already.
	*Week 8: 8-16-2010		*Week 8: 8-16-2010

---

Latest revision as of 10:21, 19 July 2010

Timeline for Team Analog

• Week 1: 6-28-2010
  • [x] Get parts and competent cells.
  • [x] Culture parts.
  • [x] Miniprep parts/make stocks.
  • [_] Characterize parts (promoters).

• Week 2: 7-05-2010
  • [x] Restriction digest parts.
  • [x] PCR superfolded GFP reporter.
  • [x] Gel extract parts.
  • [_] Order genes for kinases, phosphatases, and transcription factors.
  • [_] Characterize parts (promoters).

• Week 3: 7-12-2010
  • [x] Ligate parts.
  • [x] Order primers for small parts and promoters.
  • [x] Prepare MDV presentation and get money (hopefully).
  • [x] Order genes for kinases, phosphatases, and transcription factors.
  • [_] Streak BW27783 and culture to lose its current plasmid.
  • [_] Characterize parts (promoters).

• Week 4: 7-19-2010
  • [_] Receive primers and build promoters.
  • [_] Streak BW27783 and culture to lose its current plasmid.
  • [_] Transform ligations and conduct sequencing.
  • [_] Characterize parts (promoters).
  • [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
  • [_] Order more primers (degradation tags, truncated proteins, etc.).

• Week 5: 7-26-2010
  • [_] Receive protein parts and finish with assembly.
  • [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
  • [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)

• Week 6: 8-02-2010
  • [_] Improve design.

• Week 7: 8-09-2010
  • [_] Win iGEM. Already.

• Week 8: 8-16-2010

• Week 9: 8-23-2010

• Week 10: 7-30-2010
  • NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon.