Team:Stanford/Notebook/Timeline/Team Analog
From 2010.igem.org
(Difference between revisions)
(→Timeline for Team Analog) |
(→Timeline for Team Analog) |
||
(3 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
==Timeline for Team Analog== | ==Timeline for Team Analog== | ||
- | *Week 1 | + | *Week 1: 6-28-2010 |
+ | **[x] Get parts and competent cells. | ||
+ | **[x] Culture parts. | ||
+ | **[x] Miniprep parts/make stocks. | ||
+ | **[_] Characterize parts (promoters). | ||
+ | |||
+ | *Week 2: 7-05-2010 | ||
+ | **[x] Restriction digest parts. | ||
+ | **[x] PCR superfolded GFP reporter. | ||
+ | **[x] Gel extract parts. | ||
+ | **[_] Order genes for kinases, phosphatases, and transcription factors. | ||
+ | **[_] Characterize parts (promoters). | ||
+ | |||
+ | *Week 3: 7-12-2010 | ||
+ | **[x] Ligate parts. | ||
+ | **[x] Order primers for small parts and promoters. | ||
+ | **[x] Prepare MDV presentation and get money (hopefully). | ||
+ | **[x] Order genes for kinases, phosphatases, and transcription factors. | ||
+ | **[_] Streak BW27783 and culture to lose its current plasmid. | ||
+ | **[_] Characterize parts (promoters). | ||
+ | |||
+ | *Week 4: 7-19-2010 | ||
+ | **[_] Receive primers and build promoters. | ||
+ | **[_] Streak BW27783 and culture to lose its current plasmid. | ||
+ | **[_] Transform ligations and conduct sequencing. | ||
+ | **[_] Characterize parts (promoters). | ||
+ | **[_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?). | ||
+ | **[_] Order more primers (degradation tags, truncated proteins, etc.). | ||
+ | |||
+ | *Week 5: 7-26-2010 | ||
+ | **[_] Receive protein parts and finish with assembly. | ||
+ | **[_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro). | ||
+ | **[_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?) | ||
+ | |||
+ | *Week 6: 8-02-2010 | ||
+ | **[_] Improve design. | ||
+ | |||
+ | *Week 7: 8-09-2010 | ||
+ | **[_] Win iGEM. Already. | ||
+ | |||
+ | *Week 8: 8-16-2010 | ||
** | ** | ||
- | + | ||
- | + | *Week 9: 8-23-2010 | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | *Week 8 | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
** | ** | ||
+ | |||
+ | *Week 10: 7-30-2010 | ||
+ | **NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon. |
Latest revision as of 10:21, 19 July 2010
Timeline for Team Analog
- Week 1: 6-28-2010
- [x] Get parts and competent cells.
- [x] Culture parts.
- [x] Miniprep parts/make stocks.
- [_] Characterize parts (promoters).
- Week 2: 7-05-2010
- [x] Restriction digest parts.
- [x] PCR superfolded GFP reporter.
- [x] Gel extract parts.
- [_] Order genes for kinases, phosphatases, and transcription factors.
- [_] Characterize parts (promoters).
- Week 3: 7-12-2010
- [x] Ligate parts.
- [x] Order primers for small parts and promoters.
- [x] Prepare MDV presentation and get money (hopefully).
- [x] Order genes for kinases, phosphatases, and transcription factors.
- [_] Streak BW27783 and culture to lose its current plasmid.
- [_] Characterize parts (promoters).
- Week 4: 7-19-2010
- [_] Receive primers and build promoters.
- [_] Streak BW27783 and culture to lose its current plasmid.
- [_] Transform ligations and conduct sequencing.
- [_] Characterize parts (promoters).
- [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
- [_] Order more primers (degradation tags, truncated proteins, etc.).
- Week 5: 7-26-2010
- [_] Receive protein parts and finish with assembly.
- [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
- [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)
- Week 6: 8-02-2010
- [_] Improve design.
- Week 7: 8-09-2010
- [_] Win iGEM. Already.
- Week 8: 8-16-2010
- Week 9: 8-23-2010
- Week 10: 7-30-2010
- NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon.