Team:Cambridge/BioluminescenceLinks

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=Bioluminescence=
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{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Rough notes: Bioluminescence}}
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==Emily's Section==
==Emily's Section==
*Spectra:
*Spectra:
**Spectral Emissions of different species - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Different Species Spectra]
**Spectral Emissions of different species - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Different Species Spectra]
**Fluorescent Protein Spectra - [http://jcs.biologists.org/cgi/reprint/114/5/837 Spectra]
**Fluorescent Protein Spectra - [http://jcs.biologists.org/cgi/reprint/114/5/837 Spectra]
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**Small amount of blue-shift - [http://pubs.acs.org/doi/pdfplus/10.1021/bi7015052 Blue Shift]
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**More blue-shift - [http://www.conncoll.edu/ccacad/zimmer/GFP-ww/Research.html Blue Shift 2]
*Metridia: (luciferase requires CBP Protein)
*Metridia: (luciferase requires CBP Protein)
**Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
**Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
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***One light quantum emitted for every luciferin molecule oxidized
***One light quantum emitted for every luciferin molecule oxidized
**CoA stimulation - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B73G9-47G3TGV-1S9-1&_cdi=11521&_user=1495569&_pii=0006300258903810&_orig=search&_coverDate=12%2F31%2F1958&_sk=999729999&view=c&wchp=dGLbVzb-zSkzV&md5=4420b38ef24aba8ca23e0b8ce2b3a953&ie=/sdarticle.pdf CoA in Luminescence]
**CoA stimulation - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B73G9-47G3TGV-1S9-1&_cdi=11521&_user=1495569&_pii=0006300258903810&_orig=search&_coverDate=12%2F31%2F1958&_sk=999729999&view=c&wchp=dGLbVzb-zSkzV&md5=4420b38ef24aba8ca23e0b8ce2b3a953&ie=/sdarticle.pdf CoA in Luminescence]
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**D-cysteine powerfully inhibits E.Coli growth [http://www.ncbi.nlm.nih.gov/pubmed/11527960 D-cysteine]
*Aequorin:
*Aequorin:
**Regeneration of Aequorin in ''Aequorea Aequorea'' jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
**Regeneration of Aequorin in ''Aequorea Aequorea'' jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
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**Photinus Pyralis (firefly) spectrum, 562nm - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Reference]
**Photinus Pyralis (firefly) spectrum, 562nm - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Reference]
**Different Coloured Firefly Luciferases - [http://peds.oxfordjournals.org/cgi/reprint/18/12/581 Colours]
**Different Coloured Firefly Luciferases - [http://peds.oxfordjournals.org/cgi/reprint/18/12/581 Colours]
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**Small amount of blue-shift - [http://pubs.acs.org/doi/pdfplus/10.1021/bi7015052 Blue Shift]
 
*Gaussia Princeps (copepod):
*Gaussia Princeps (copepod):
**GLuc - [http://aem.asm.org/cgi/content/abstract/71/7/3427 GLuc]
**GLuc - [http://aem.asm.org/cgi/content/abstract/71/7/3427 GLuc]
**100 Times Brighter than P. Pyrilus!! - [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth979.html So bright]
**100 Times Brighter than P. Pyrilus!! - [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth979.html So bright]
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**GLuc expression in E. Coli [http://pubs.acs.org/doi/pdfplus/10.1021/ac025742k E.GLuc]
==Theo's section==
==Theo's section==
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===Firefly===
===Firefly===
* The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf
* The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf
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===Biobricks related to light===
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===Luciferases====
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*Coelentrazine using
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**[http://partsregistry.org/Part:BBa_J52008 Renilla luciferase]
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*Firefly luciferin using
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**[http://partsregistry.org/Part:BBa_I712019 Firefly luciferase]
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*Produces all parts of pathway:
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**[http://partsregistry.org/Part:BBa_J70008 Lux operon from photorhabdus]
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*Click beetle:
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**[http://partsregistry.org/Part:BBa_J70005 Red]
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====Fluorescent proteins====
==Bill's section==
==Bill's section==
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* Don't use luciferinol, it r teh suckage
* Don't use luciferinol, it r teh suckage
* [http://jeb.biologists.org/cgi/reprint/137/1/39.pdf Glowing fish what?]
* [http://jeb.biologists.org/cgi/reprint/137/1/39.pdf Glowing fish what?]
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* Some actual numbers on luciferin [http://pubs.acs.org/doi/abs/10.1021/bi060475o]
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* D-cysteine is bad for e-coli [http://www.jbc.org/content/276/44/40864.full]
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* luminaze - good for brighter and longer wavelength light [http://pubs.acs.org/doi/abs/10.1021/bi00327a026]
==Will's section==
==Will's section==
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==Ben's section==
==Ben's section==
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* Flashlight fish symbiont luciferase genes have been sequenced but cannot be sucessfully expressed in E. Coli
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* Flashlight fish symbiont luciferase genes have been sequenced but cannot be successfully expressed in E. Coli
* Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
* Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
* The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
* The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
* Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART
* Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART
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[[Image:luciferin.png]]
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[[Image:luciferin.png|600px]]
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* L-luciferin can be oxidised to produce light but with half the efficiency http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1217473/
==Paul's section==
==Paul's section==
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* [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THS-4HWX8NX-C&_user=1495569&_coverDate=02%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402624057&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b2ccca6fb4c15f90120534c45c40bc8e Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase]
* [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THS-4HWX8NX-C&_user=1495569&_coverDate=02%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402624057&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b2ccca6fb4c15f90120534c45c40bc8e Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase]
* Biobrick for the Firefly luciferase for Slovenia '07: http://partsregistry.org/Part:BBa_I712019:Design
* Biobrick for the Firefly luciferase for Slovenia '07: http://partsregistry.org/Part:BBa_I712019:Design
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* [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-46RN5P2-H&_user=1495569&_coverDate=07%2F10%2F2002&_rdoc=17&_fmt=high&_orig=browse&_srch=doc-info%28%23toc%234941%232002%23997059998%23338594%23FLA%23display%23Volume%29&_cdi=4941&_sort=d&_docanchor=&_ct=31&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=0522f6433170194d4a35ad67a99dab7f Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis]
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Latest revision as of 23:45, 10 August 2010

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