Team:EPF Lausanne/Protocols

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(Lab Protocols)
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== Lab Protocols ==
== Lab Protocols ==
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
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*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol]
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*[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH]
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*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol]
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*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel]
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*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol]
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*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production]
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*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol]
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*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ]
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* adapt ph
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*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
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* electrophoresis
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*[https://static.igem.org/mediawiki/igem.org/8/82/Prot_growing_asaia.pdf Growing Asaia]
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* UV imaging
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*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
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* [https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
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*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
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* PCR  
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* Miniprep: use Miniprep Kit
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*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
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* Using the plate reader
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*[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ]
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* Production of (agar) plates
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*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof]
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* Asaia Maxiprep
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* Purification: use Purification Kit
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* Asaia Miniprep
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*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
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*[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]
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*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion]
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*[https://static.igem.org/mediawiki/2010/6/68/Prot_WesternBlot.pdf Western Blot]
Media Recipes
Media Recipes
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*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
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<b>Syntax:</b>convention if you want to upload a new protocol<br\>
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When uploading a file it please rename it in the following way: <i>prot_yourProcedureName.yourExtension</i>.
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<b>Syntax: </b>convention if you want to upload a new protocol  <br\>
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{{EPFL_2010_bottom_wrap}}
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When uploading a file it please rename it in the following way: <i> prot_yourProcedureName.yourExtension</i>.
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</div>

Latest revision as of 23:15, 27 October 2010



Asaia protocols.png

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Media Recipes

Syntax:convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.

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