Team:EPF Lausanne/Protocols

From 2010.igem.org

(Difference between revisions)
(Lab Protocols)
(Lab Protocols)
 
(51 intermediate revisions not shown)
Line 1: Line 1:
 +
{{EPFL_2010_2}}
 +
<html>
 +
<head> <style>
 +
 +
.EPFL_content
 +
{
 +
    margin-right:190px;
 +
}
 +
 +
</style></head>
 +
 +
<div style="position: absolute; left: 0px; top: 60px; height: 140px; width: 975px;">
 +
<img alt="" src="https://static.igem.org/mediawiki/2010/7/70/EPFL_Title_2_Protocol.png"/></div>
 +
 +
<div style="position: absolute; left: 435px; top: 11px; height: 85px; width: 125px;">
 +
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook">
 +
<img alt="" height="80" src="https://static.igem.org/mediawiki/2010/b/b9/EPFL_Menu_2_Notebook.png" width="125" class="auto-style1" /></a></div>
 +
 +
</html>
 +
 +
{{EPFL_sub_menu_notebook}}
 +
 +
 +
<div CLASS="EPFL_content">
 +
 +
<center>
 +
[[Image: asaia_protocols.png]]
 +
</center>
 +
== Lab Protocols ==
== Lab Protocols ==
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
-
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol]
+
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH]
-
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol]
+
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel]
-
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol]
+
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production]
-
* adapt ph
+
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ]
-
* electrophoresis
+
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
-
* UV imaging
+
*[https://static.igem.org/mediawiki/igem.org/8/82/Prot_growing_asaia.pdf Growing Asaia]
-
* [https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
+
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
-
* PCR  
+
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
-
* Using the plate reader
+
* Miniprep: use Miniprep Kit
-
* Production of (agar) plates
+
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
-
* Asaia Maxiprep
+
*[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ]
-
* Asaia Miniprep
+
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof]
 +
* Purification: use Purification Kit
 +
*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
 +
*[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]
 +
*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion]
 +
*[https://static.igem.org/mediawiki/2010/6/68/Prot_WesternBlot.pdf Western Blot]
Media Recipes
Media Recipes
Line 21: Line 55:
*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
-
<b>Syntax: </b>convention if you want to upload a new protocol <br\>
+
<b>Syntax:</b>convention if you want to upload a new protocol<br\>
-
When uploading a file it please rename it in the following way: <i> prot_yourProcedureName.yourExtension</i>.
+
When uploading a file it please rename it in the following way: <i>prot_yourProcedureName.yourExtension</i>.
 +
 
 +
{{EPFL_2010_bottom_wrap}}
 +
</div>

Latest revision as of 23:15, 27 October 2010



Asaia protocols.png

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Media Recipes

Syntax:convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.

wrap bottom