Team:KAIST-Korea/Notebook/Memo/Info
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+ | == <span style=font-size:20px> <b> Information </b> </span> == | ||
+ | <br> | ||
+ | <span style=font-size:15px> <b> AP1 binding site </b> </span><br> | ||
+ | AP1 binding site is promoter site where Activation Protein 1 binds to regulate gene expression. <br> | ||
+ | The known sequence referenced from Wikipedia is TGAGTCA. This site is known as an essential part <br> | ||
+ | for regulation of COL1A2 promoter activity by transforming growth factor 2. | ||
+ | Due to property of AP1, only expressed naturally in human, interacts directly with DNA in nucleus,<br> | ||
+ | and well known promoter sequence, our team decided to use it as core regulator in DiscoverY project. <br> | ||
+ | Regardless of orginal biological function of AP1 and its binding site, this region is | ||
+ | now used to activate its own promoter, which is linked to GFP gene. | ||
+ | When AP1 is expressed and activated, this protein initiates gene expression <br> | ||
+ | and linked gene, GFP in our case, is expressed with fluorescent signal which means detection of and antigen. <br> | ||
+ | <br> | ||
<span style=font-size:15px> <b> Malaria </b> </span> | <span style=font-size:15px> <b> Malaria </b> </span> | ||
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But, it is failed because it is very ineffective although it is possible theoretically.<br> | But, it is failed because it is very ineffective although it is possible theoretically.<br> | ||
Todays research, they find antigen which make antibody attached parasite membrane or surface protein. | Todays research, they find antigen which make antibody attached parasite membrane or surface protein. | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> Aerobic bacteria </b> </span> | ||
+ | <br> | ||
+ | Some bacteria react the magnetic field. These bacteria, in magnetic field, stand in a line.<br> | ||
+ | Not only these but also many animals have some mechanisms which recognize magnetic area.<br> | ||
+ | In addition, aerobic or anaerobic microbes line when they are stimulted by oxygen. | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> DDS system using HNMT </b> </span> | ||
+ | <br> | ||
+ | Drug Delivery System(DDS) using virus has tissue specificity. | ||
+ | For example, Coronavirus usually infect Upper respiratory tracts.<br> | ||
+ | HNMT(histamine N-methylatransferase) is a enzyme used in metabolic of histamine and inactivate histamine to deliver methyl.<br> | ||
+ | There are two kinds of enzyme, one is DAO, the other is HNMT.<br> | ||
+ | Because DAO has a copper on its sturucture, so it is hard to sythesize it.<br> | ||
+ | Fortunately, HNMT is a single strand protein which is composed just 290 anmino acid. | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> METHYLATION PHARMACOGENETICS </b> </span> | ||
+ | <br> | ||
+ | METHYLATION PHARMACOGENETICS<br> | ||
+ | Catechol O-Methyltransferase,Thiopurine Methyltransferase, and Histamine N-Methyltransferase<br> | ||
+ | Richard M. Weinshilboum, Diane M. Otterness,and Carol L. Szumlanski<br> | ||
+ | Department of Pharmacology, Mayo Medical School/Mayo Clinic/Mayo Foundation,Rochester, Minnesota 55905<br> | ||
+ | e-mail: weinshilboum.richard@mayo.edu<br> | ||
+ | KEY WORDS: genetic polymorphisms, thiol methyltransferase, nicotinamide N-methyltransferase, thioether methyltransferase, phenylethanolamine N-methyltransferase<br><br> | ||
+ | This paper is for genetic polymorphisms related of methylation.<br> | ||
+ | It treats various mythytrasferase mechanisms including HNMT<br><br> | ||
+ | |||
+ | <span style=font-size:15px> <b> The Result of BLAST </b> </span><br> | ||
+ | I put the sequences of Dong Chan in BioBrick DB.<br> | ||
+ | There is no corresponding brick as we expected.<br> | ||
+ | It can be good news but on the other hand, we should search spike protein sequences. <br> | ||
+ | There were some partial analogous sequences, but those are not related to our sequences in function.<br> | ||
+ | I’ll post the result of BLAST parsing for feedback | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> E.Coli OMV </b> </span><br> | ||
+ | Maybe we could use hemolysin which Stanford team used. <br> | ||
+ | The technology transporting antigen for vaccine using Bacterial Outer Membrane Vescicle(OMV) is published in the February <br>issue of PNAS. See the attached file.<br> | ||
+ | P.S. There is a similar thesis in J. Mol. Biol. It shows the functional improvement of OMV.<br> | ||
+ | It also is attached.<br> | ||
+ | We may be able to use vesicle in E.coli. | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> STAT1 & JAK1 </b> </span> | ||
+ | <br> | ||
+ | STAT1 information | ||
+ | :http://www.uniprot.org/uniprot/P42224 | ||
+ | |||
+ | Human JAK1 information | ||
+ | :http://www.uniprot.org/uniprot/P23458<br><br> | ||
+ | <span style=font-size:15px> <b> STAT's Promoter </b> </span> | ||
+ | <br> | ||
+ | STAT1 homodimers are involved in type II interferon signalling, and binds to the GAS (Interferon-Gamma Activated Sequence) promoter to induce expression of ISG (Interferon Stimulated Genes). | ||
+ | In type I interferon signaling, STAT1-STAT2 heterodimer combines with IRF9 (Interferon Response Factor) to form ISGF3 (Interferon Stimulated Gene Factor), which binds to the ISRE (Interferon Stimulated Response Element) promoter to induce ISG expression. | ||
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Latest revision as of 05:03, 17 October 2010
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