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Team:Michigan/Kevin's Notebook
From 2010.igem.org
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Poured agar plates. | Poured agar plates. | ||
Created 500 mL of agar, poured 20 plates. | Created 500 mL of agar, poured 20 plates. | ||
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| + | ==7/15/10== | ||
| + | Performed digest on certain biobrick parts. | ||
| + | Performed gel electrophoresis to determine validity of parts. | ||
| + | *Most of the parts were the expected length. | ||
Latest revision as of 23:32, 15 July 2010
6/28/2010
Made a 500 mL batch of LB broth
- When pouring in distilled water, pour a few mL at a time to avoid clumping.
- It takes 20g of powder to make 1 L of broth
Sterilized broth using the autoclave
- The temperature setting on the autoclave is off by a little bit
- Set dial 2 notches below 134 °C.
6/29/2010
Started growing E. coli K12 cultures
- Poured 2 mL of LB broth into a Falcon tube
- Used strain of K12 from Dr. Lin's freezer
- Placed in incubator shaker for 24 hrs.
Added and inventoried supplies from Dr. Pinto's lab.
- Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
- Chemical wastes must be cleaned by calling OSEH.
7/14/10
Poured agar plates. Created 500 mL of agar, poured 20 plates.
7/15/10
Performed digest on certain biobrick parts. Performed gel electrophoresis to determine validity of parts.
- Most of the parts were the expected length.
