"
Team:TU Delft/24 April 2010 content
From 2010.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | + | =Session 8= | |
Now it’s not if we like the idea anymore (otherwise it wouldn’t be a chosen idea), but how could we accomplish it? How feasible is it? What BioBricks do we expect to make with this? | Now it’s not if we like the idea anymore (otherwise it wouldn’t be a chosen idea), but how could we accomplish it? How feasible is it? What BioBricks do we expect to make with this? | ||
| - | + | ===1. Oil removal=== | |
* Hydrocarbon degradation | * Hydrocarbon degradation | ||
* Oil sand processing gives wastewater with allot of oil (bitumen) => Convert this wastewater into something valuable | * Oil sand processing gives wastewater with allot of oil (bitumen) => Convert this wastewater into something valuable | ||
| Line 8: | Line 8: | ||
Split into 3 teams: | Split into 3 teams: | ||
| - | + | ||
| - | + | '''Hydrocarbon degradation''' | |
* Maybe start with Benzoic acid & Alkenes? | * Maybe start with Benzoic acid & Alkenes? | ||
* Probably the most work of all the teams, but others could help once they are done | * Probably the most work of all the teams, but others could help once they are done | ||
| Line 15: | Line 15: | ||
* Be careful whether the intermediates may be toxic | * Be careful whether the intermediates may be toxic | ||
* Sugar metabolism is no longer necessary in E.coli | * Sugar metabolism is no longer necessary in E.coli | ||
| - | + | ||
| + | '''Salt tolerance''' | ||
* Give the other team a strain of E.coli that can handle salt. | * Give the other team a strain of E.coli that can handle salt. | ||
| - | |||
* Biobrick 2009; not clear whether it works or not? | * Biobrick 2009; not clear whether it works or not? | ||
| - | + | ||
| + | '''Overcoming mass transfer''' | ||
* Adding an emulsifier to make the oil soluble? | * Adding an emulsifier to make the oil soluble? | ||
| - | + | * Production of hydrophilic/hydrophobic peptide? | |
| - | * Production of hydrophilic/hydrophobic peptide | + | |
| - | + | '''Extra features''' | |
* We are making allot of acetyl CoA, so why not produce something useful? | * We are making allot of acetyl CoA, so why not produce something useful? | ||
| - | * Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick) | + | * Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick), PHA/PHB |
| - | + | ||
| - | + | '''Other points''' | |
| - | + | ||
* Money / many biobricks / lot of literature | * Money / many biobricks / lot of literature | ||
* Is E.coli the bug to go with? | * Is E.coli the bug to go with? | ||
| Line 37: | Line 37: | ||
'''Vote: Yes (8/8)''' | '''Vote: Yes (8/8)''' | ||
| - | + | ===2. Algae bloom /nitrate reduction=== | |
| - | + | ||
* A mutated FNR can induce the nitrate consumption under aerobic conditions | * A mutated FNR can induce the nitrate consumption under aerobic conditions | ||
| - | |||
* Biobrick for nitrite sensor | * Biobrick for nitrite sensor | ||
* E.coli would probably be outcompeted by the algae? Depends on the carbon source | * E.coli would probably be outcompeted by the algae? Depends on the carbon source | ||
| - | |||
* Some enzymes will be inhibited by oxygen | * Some enzymes will be inhibited by oxygen | ||
'''Vote: No (0/8)''' | '''Vote: No (0/8)''' | ||
| - | + | ===3. Appetite inhibitor=== | |
* Caerulein | * Caerulein | ||
* Combination with circadian clock for times of production and non-production? | * Combination with circadian clock for times of production and non-production? | ||
| - | |||
* Regulation is definitely necessary | * Regulation is definitely necessary | ||
* Couple it to blood sugar levels? Not really possible to sensor something in the blood from within the gut? | * Couple it to blood sugar levels? Not really possible to sensor something in the blood from within the gut? | ||
'''Vote: No (3/8)''' | '''Vote: No (3/8)''' | ||
| - | + | ===4. Circadian Clocks / oscillators=== | |
* Oscillations are easy to come by | * Oscillations are easy to come by | ||
| - | |||
* Tool for another project? | * Tool for another project? | ||
* Hard to accurately predict the actual frequency; could only occur through true labwork | * Hard to accurately predict the actual frequency; could only occur through true labwork | ||
Latest revision as of 19:04, 2 August 2010
Contents |
Session 8
Now it’s not if we like the idea anymore (otherwise it wouldn’t be a chosen idea), but how could we accomplish it? How feasible is it? What BioBricks do we expect to make with this?
1. Oil removal
- Hydrocarbon degradation
- Oil sand processing gives wastewater with allot of oil (bitumen) => Convert this wastewater into something valuable
- Salinity, pH, mass transfer problems
Split into 3 teams:
Hydrocarbon degradation
- Maybe start with Benzoic acid & Alkenes?
- Probably the most work of all the teams, but others could help once they are done
- Most likely an aerobic process (most likely none of the enzymes are oxygen sensitive)
- Be careful whether the intermediates may be toxic
- Sugar metabolism is no longer necessary in E.coli
Salt tolerance
- Give the other team a strain of E.coli that can handle salt.
- Biobrick 2009; not clear whether it works or not?
Overcoming mass transfer
- Adding an emulsifier to make the oil soluble?
- Production of hydrophilic/hydrophobic peptide?
Extra features
- We are making allot of acetyl CoA, so why not produce something useful?
- Biofuel? Propanol/ethanol/biodiesel? There are some biobricks for this… but no Exporter? (Biobrick), PHA/PHB
Other points
- Money / many biobricks / lot of literature
- Is E.coli the bug to go with?
- We don’t necessarily have to put all 4 projects into one strain, but we can start with 4 and maybe if we have time we could try to.
- Good project that represents Delft (mass transfer etc.)
- There are no biobricks for hydrocarbon degradation!
Vote: Yes (8/8)
2. Algae bloom /nitrate reduction
- A mutated FNR can induce the nitrate consumption under aerobic conditions
- Biobrick for nitrite sensor
- E.coli would probably be outcompeted by the algae? Depends on the carbon source
- Some enzymes will be inhibited by oxygen
Vote: No (0/8)
3. Appetite inhibitor
- Caerulein
- Combination with circadian clock for times of production and non-production?
- Regulation is definitely necessary
- Couple it to blood sugar levels? Not really possible to sensor something in the blood from within the gut?
Vote: No (3/8)
4. Circadian Clocks / oscillators
- Oscillations are easy to come by
- Tool for another project?
- Hard to accurately predict the actual frequency; could only occur through true labwork
- Frequency would be dependent on the concentrations of the compounds as well as the growth rates for these also determine the degradation etc.
- How could you change affinity constants? (Kai system)
- Biobrick for Kai is already available, but how can you influence the frequency without trial-and-error?
- There is literature that could help make a model…
Vote: No (3/8)
