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Team:Michigan/Josh's Notebook
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| + | {{Michigan Header}} | ||
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7/10/2010 | 7/10/2010 | ||
13:00 | 13:00 | ||
| - | + | • digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1. | |
| Line 8: | Line 11: | ||
9:00 | 9:00 | ||
| - | + | • inoculate competent cell culture | |
| + | |||
| + | • harvest I719015, K145001, K117008, K117002, K103006 from registry | ||
| + | |||
| + | |||
| + | 7/15/2010 | ||
| + | 4:30 | ||
| + | |||
| + | • streak plate INP NC sample | ||
| + | |||
| + | |||
| + | 7/18/2010 | ||
| + | 5:00 | ||
| + | |||
| + | • miniprep INP NC sample | ||
| + | |||
| + | |||
| + | 7/21/2010 | ||
| + | 6:00 | ||
| + | |||
| + | • digest INP NC with EcoRI and SpeI | ||
| + | |||
| + | |||
| + | 7/23/2010 | ||
| + | 9:00 | ||
| + | |||
| + | • gel electrophoresis of all biobricks | ||
| + | |||
| + | [[Image:biobrickgel.jpg|300px]] | ||
| + | [[Image:ladder.jpg|100px]] | ||
| + | |||
| + | lane 1 - ladder | ||
| + | |||
| + | lane 2 - INP NC sample 1 (K265008) | ||
| + | |||
| + | lane 3 - INP NC sample 2 (K265008) | ||
| + | |||
| + | lane 4 - T7 GFPmut3b (I719015) | ||
| + | |||
| + | lane 5 - T7 Promoter (I719005) | ||
| + | |||
| + | lane 6 - T7 RNA polymerase (K145001) | ||
| + | |||
| + | lane 7 - pLsrA-YFP (K117008) | ||
| + | |||
| + | lane 8 - LsrA promoter (K117002) | ||
| + | |||
| + | lane 9 - OmpA (K103006) | ||
| + | |||
| + | |||
| + | 7/25/2010 | ||
| + | 5:00 | ||
| + | |||
| + | • aliquot INP samples and primers for sequencing | ||
| + | |||
| + | 7/28/2010 | ||
| + | 4:00-6:00 pm | ||
| + | |||
| + | Gel electrophoresis of GFP, INP-NC, Linker, and OmpA Biobricks | ||
| - | + | (Pictures / analysis go here) | |
Latest revision as of 22:35, 28 July 2010
7/10/2010
13:00
• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.
7/13/2010
9:00
• inoculate competent cell culture
• harvest I719015, K145001, K117008, K117002, K103006 from registry
7/15/2010
4:30
• streak plate INP NC sample
7/18/2010
5:00
• miniprep INP NC sample
7/21/2010
6:00
• digest INP NC with EcoRI and SpeI
7/23/2010
9:00
• gel electrophoresis of all biobricks
lane 1 - ladder
lane 2 - INP NC sample 1 (K265008)
lane 3 - INP NC sample 2 (K265008)
lane 4 - T7 GFPmut3b (I719015)
lane 5 - T7 Promoter (I719005)
lane 6 - T7 RNA polymerase (K145001)
lane 7 - pLsrA-YFP (K117008)
lane 8 - LsrA promoter (K117002)
lane 9 - OmpA (K103006)
7/25/2010
5:00
• aliquot INP samples and primers for sequencing
7/28/2010 4:00-6:00 pm
Gel electrophoresis of GFP, INP-NC, Linker, and OmpA Biobricks
(Pictures / analysis go here)
