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Team:Panama/14 October 2010
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We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). | We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). | ||
| - | Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert ( | + | Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert (Rh1AB) or not. |
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'''Miniprep Protocol:''' | '''Miniprep Protocol:''' | ||
| - | For this procedure we used what is called a "dirty miniprep". | + | For this procedure we used what is called a "dirty miniprep". And by dirty we don't mean that we did it with unclean utensils nor did we do it after coming from a soccer game; it means that the reagents were prepared in the lab and they weren't obtained from a commercial kit. |
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Latest revision as of 03:49, 28 October 2010
October 14
Prepared Ampicillin stock (50 mg/ml)
We did a miniprep on the 11 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out). Then, we ran a gel of 1% agarose to see if we could see a difference in the size of the linear band of the plasmid to be able to determine if it contains the insert (Rh1AB) or not.
Miniprep Protocol:
For this procedure we used what is called a "dirty miniprep". And by dirty we don't mean that we did it with unclean utensils nor did we do it after coming from a soccer game; it means that the reagents were prepared in the lab and they weren't obtained from a commercial kit.
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