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Team:TU Delft/1 July 2010 content
From 2010.igem.org
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| - | + | =Lab Work= | |
| + | ==''Pseudomonas Putida'' GPO1== | ||
The second day of the grow process of ''Pseudomonas Putida'' GPO1 | The second day of the grow process of ''Pseudomonas Putida'' GPO1 | ||
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| - | |Sample | + | |'''Sample''' |
| - | | | + | |'''Medium''' |
| - | |day 1 | + | |'''day 1 = 30 June''' |
| - | |day 2 | + | |'''day 2 = 1 July''' |
|- | |- | ||
|no cells | |no cells | ||
| - | |E2 | + | |E2 without carbon |
|0.00 | |0.00 | ||
|0.00 | |0.00 | ||
|- | |- | ||
|no cells | |no cells | ||
| - | |LB | + | |LB |
|0.00 | |0.00 | ||
|0.00 | |0.00 | ||
| Line 54: | Line 55: | ||
|} | |} | ||
| - | * average of 3 colonies | + | '''*''' average of 3 colonies |
| + | |||
| + | ''Pseudomonas Putida'' GPO1 can grow on 10% octane as carbon source. | ||
| + | |||
| + | ==Competent cells== | ||
| + | Top10 cells were cultivated for making competent cells. | ||
Latest revision as of 19:21, 2 August 2010
Lab Work
Pseudomonas Putida GPO1
The second day of the grow process of Pseudomonas Putida GPO1
| Sample | Medium | day 1 = 30 June | day 2 = 1 July |
| no cells | E2 without carbon | 0.00 | 0.00 |
| no cells | LB | 0.00 | 0.00 |
| no cells | E2 + 1% LB | 0.00 | 0.01 |
| Pseudomonas Putida GPO1 * | E2 + 1% LB | 0.01 | 0.05 |
| no cells | E2 + 10% LB | 0.00 | 0.00 |
| Pseudomonas Putida GPO1 * | E2 + 10% LB | 0.34 | 0.19 |
| no cells | E2 + 1% octane | 0.00 | 0.00 |
| Pseudomonas Putida GPO1 * | E2 + 1% octane | 0.00 | 0.00 |
| Pseudomonas Putida GPO1 * | E2 + 10% octane | 0.29 | 0.18 |
* average of 3 colonies
Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.
Competent cells
Top10 cells were cultivated for making competent cells.
