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Team:TU Delft/18 June 2010 content
From 2010.igem.org
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| + | =Cleaning= | ||
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | ||
We also booked a hotel in Boston! | We also booked a hotel in Boston! | ||
| + | =Lab work= | ||
| + | |||
| + | ==Emulsifier== | ||
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Pieter worked on the emulsifying activity assay. Four samples were tested: | Pieter worked on the emulsifying activity assay. Four samples were tested: | ||
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| - | | | + | |'''#''' |
| - | |Amount of SDS (%) | + | |'''Amount of SDS (%)''' |
| - | |Hexalkane (mL) | + | |'''Hexalkane (mL)''' |
|- | |- | ||
|1 | |1 | ||
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|0.02 | |0.02 | ||
|} | |} | ||
| - | |||
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml. | 2. Tris buffer (pH 8) was added to a total volume of 7.5 ml. | ||
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4. Measure absorbance OD600 | 4. Measure absorbance OD600 | ||
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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| - | | | + | |'''#''' |
| - | |A600 | + | |'''A600''' |
|- | |- | ||
|1 | |1 | ||
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|0.2 | |0.2 | ||
|} | |} | ||
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| + | ==Biobricks== | ||
| + | By Hugo | ||
| + | |||
| + | I've got some colonies in the plates, however there's contamination with some yellow colonies :s | ||
| + | Probably, we will have to prepare a new stock of competent cells. | ||
Latest revision as of 08:28, 26 August 2010
Contents |
Cleaning
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
We also booked a hotel in Boston!
Lab work
Emulsifier
Pieter worked on the emulsifying activity assay. Four samples were tested:
1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
| # | Amount of SDS (%) | Hexalkane (mL) |
| 1 | 0 | 0 |
| 2 | 0 | 0.02 |
| 3 | 0.01 | 0.02 |
| 4 | 1 | 0.02 |
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.
3. Shake 1 hour with 200 strokes/min at 30°C
4. Measure absorbance OD600
| # | A600 |
| 1 | 0 |
| 2 | 0 |
| 3 | 0.4 |
| 4 | 0.2 |
Biobricks
By Hugo
I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.
