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Team:Panama/11 October 2010
From 2010.igem.org
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==='''October 11 '''=== | ==='''October 11 '''=== | ||
| - | Today we | + | Today we measure the concentration of the purified PCR product and the result was 107ug/ml. |
We also determined that for the cloning using the pGEMT Easy Kit it is necessary to use a ratio of 3:1. 3 being the vector amount and 1 being the insert amount. | We also determined that for the cloning using the pGEMT Easy Kit it is necessary to use a ratio of 3:1. 3 being the vector amount and 1 being the insert amount. | ||
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Electrophoresis of the ligation product: Terminator, GFP | Electrophoresis of the ligation product: Terminator, GFP | ||
| - | 1% agarose gel, TBE 1X, 2. | + | 1% agarose gel, TBE 1X, 2.5ul EtBr. We decided to leave well 3 empty just so it'd look pretty, pretty and it does! |
| - | [[Image: | + | [[Image:Untitled27.JPG|250px|thumb|left|alt text]] |
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| - | [[Image: | + | [[Image:UntitledGel11_Oct_2.JPG|200px|thumb|left|alt text]] |
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742 GFP | 742 GFP | ||
| - | + | 3948 bp | |
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Latest revision as of 03:18, 28 October 2010
October 11
Today we measure the concentration of the purified PCR product and the result was 107ug/ml.
We also determined that for the cloning using the pGEMT Easy Kit it is necessary to use a ratio of 3:1. 3 being the vector amount and 1 being the insert amount.
Now we are cloning with the pGEMT Easy Kit
Electrophoresis of the ligation product: Terminator, GFP
1% agarose gel, TBE 1X, 2.5ul EtBr. We decided to leave well 3 empty just so it'd look pretty, pretty and it does!
3055 PsB1AC3
151 Terminator
742 GFP
3948 bp
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