Latest revision as of 19:55, 27 October 2010

As summer project our experimental work began in August, after intensive brainstorming we finnaly decide

between two options.

*Inmunoresponse using Synthetic Biology.

The final design of the expressions modules were as follow:

Next Notebook sheet is our reference for primers and ligations and for modules Assembly:

The AFP (Antifreeze Protein) was synthesized as follow:

We al ready made quimio and electro-competent cells for transformation and stored in aliquots in containers vials at -80 degrees..

We have not obtained transformats cells.

We checked out Cloramphenicol stock concentration and attempt again the transformation used PSB1C3.

The primers arrived and we proceed to amplify the modules by PCR.

The amplification was ok and we had looking for a nano spectrophotometer.

we made quantification of DNA concentrations

@@ Line 57: / Line 57: @@
 ==''Wednesday''==
-=='''We have not obtained  transformats cells.'''==
+'''We have not obtained  transformats cells.'''
-==='''We checked out the Cloramphenicol'''===
+'''We checked out Cloramphenicol stock concentration and attempt again the transformation used PSB1C3.'''
-==='''dose and attempt again the transformation using Psb1C3.'''===
 ==''Thursday''==
-==='''The primers arrived '''===
+'''The primers arrived and we proceed to amplify the modules by PCR.'''
-==='''and we proceed to amplify the modules by PCR.'''===
 [[Image:PCR.gif]]
-==='''The amplification was ok and to quantify DNA concentrations'''===
+'''The amplification was ok and we had looking for a nano spectrophotometer.'''
-==='''we had looking for a nano spectrophotometer.'''===
+'''we made quantification of DNA concentrations'''