HKU-Hong Kong/12 July 2010

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
-
{{:Team:HKU-Hong_Kong/header}}
+
{{:Team:HKU-Hong_Kong/header1}}
-
[[Image:12jul.jpg|300px|frame|double enzyme digestion of BBa_k112808 & pET28a by NotI &XbaI]]
+
<h1>Our official start:12 July 2010</h1>
<h1>Our official start:12 July 2010</h1>
After 2 weeks of training on basic techniques on biological lab work, we started our project by testing the functionality of the suicide gene.
After 2 weeks of training on basic techniques on biological lab work, we started our project by testing the functionality of the suicide gene.
Line 8: Line 7:
To obtain biopart BBa_k112808 for further modifications and combination with other bioparts in different plasmid.
To obtain biopart BBa_k112808 for further modifications and combination with other bioparts in different plasmid.
=Results=
=Results=
 +
[[Image:12jul.jpg|300px]]
 +
A gel photo was taken for detection of cut DNA fragments.
A gel photo was taken for detection of cut DNA fragments.
==BBa_k112808==
==BBa_k112808==

Latest revision as of 18:20, 27 October 2010

July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/11_July_2010&action=edit 11]
[http://2010.igem.org/HKU-Hong_Kong/12_July_2010 12] [http://2010.igem.org/HKU-Hong_Kong/13_July_2010 13] [http://2010.igem.org/HKU-Hong_Kong/14_July_2010 14] [http://2010.igem.org/HKU-Hong_Kong/15_July_2010 15] [http://2010.igem.org/HKU-Hong_Kong/16_July_2010 16] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/19_July_2010&action=edit 19] [http://2010.igem.org/HKU-Hong_Kong/20_July_2010 20] [http://2010.igem.org/HKU-Hong_Kong/21_July_2010 21] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/22_July_2010&action=edit 22] [http://2010.igem.org/HKU-Hong_Kong/23_July_2010 23] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/25_July_2010&action=edit 25]
[http://2010.igem.org/HKU-Hong_Kong/26_July_2010 26] [http://2010.igem.org/HKU-Hong_Kong/27_July_2010 27] [http://2010.igem.org/HKU-Hong_Kong/28_July_2010 28] [http://2010.igem.org/HKU-Hong_Kong/29_July_2010 29] [http://2010.igem.org/HKU-Hong_Kong/30_July_2010 30] [http://2010.igem.org/HKU-Hong_Kong/31_July_2010 31]
August
MTWTFSS
            [http://2010.igem.org/HKU-Hong_Kong/1_August_2010 1]
[http://2010.igem.org/HKU-Hong_Kong/2_August_2010 2] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/3_August_2010&action=edit 3] [http://2010.igem.org/HKU-Hong_Kong/4_August_2010 4] [http://2010.igem.org/HKU-Hong_Kong/5_August_2010 5] [http://2010.igem.org/HKU-Hong_Kong/6_August_2010 6] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/7_August_2010&action=edit 7] [http://2010.igem.org/HKU-Hong_Kong/8_August_2010 8]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/9_August_2010&action=edit 9] [http://2010.igem.org/HKU-Hong_Kong/10_August_2010 10] [http://2010.igem.org/HKU-Hong_Kong/11_August_2010 11] [http://2010.igem.org/HKU-Hong_Kong/12_August_2010 12] [http://2010.igem.org/HKU-Hong_Kong/13_August_2010 13] [http://2010.igem.org/HKU-Hong_Kong/14_August_2010 14] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/15_August_2010&action=edit 15]
[http://2010.igem.org/HKU-Hong_Kong/16_August_2010 16] [http://2010.igem.org/HKU-Hong_Kong/17_August_2010 17] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/18_August_2010&action=edit 18] [http://2010.igem.org/HKU-Hong_Kong/19_August_2010 19] [http://2010.igem.org/HKU-Hong_Kong/20_August_2010 20] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/22_August_2010&action=edit 22]
[http://2010.igem.org/HKU-Hong_Kong/23_August_2010 23] [http://2010.igem.org/HKU-Hong_Kong/24_August_2010 24] [http://2010.igem.org/HKU-Hong_Kong/25_August_2010 25] [http://2010.igem.org/HKU-Hong_Kong/26_August_2010 26] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/27_August_2010&action=edit 27] [http://2010.igem.org/HKU-Hong_Kong/28_August_2010 28] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/30_August_2010&action=edit 30] [http://2010.igem.org/HKU-Hong_Kong/31_August_2010 31]
September
MTWTFSS
    [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/6_September_2010&action=edit 6] [http://2010.igem.org/HKU-Hong_Kong/7_September_2010 7] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/8_September_2010&action=edit 8] [http://2010.igem.org/HKU-Hong_Kong/9_September_2010 9] [http://2010.igem.org/HKU-Hong_Kong/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/13_September_2010&action=edit 13] [http://2010.igem.org/HKU-Hong_Kong/14_September_2010 14] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/15_September_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/16_September_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/20_September_2010&action=edit 20] [http://2010.igem.org/HKU-Hong_Kong/21_September_2010 21] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/25_September_2010&action=edit 25] [http://2010.igem.org/HKU-Hong_Kong/26_September_2010 26]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/27_September_2010&action=edit 27] [http://2010.igem.org/HKU-Hong_Kong/28_September_2010 28] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/30_September_2010&action=edit 30]
October
MTWTFSS
        [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/1_October_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/4_October_2010&action=edit 4] [http://2010.igem.org/HKU-Hong_Kong/5_October_2010 5] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/6_October_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/7_October_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/8_October_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/9_October_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/10_October_2010&action=edit 10]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/11_October_2010&action=edit 11] [http://2010.igem.org/HKU-Hong_Kong/12_October_2010 12] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/13_October_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/14_October_2010&action=edit 14] [http://2010.igem.org/HKU-Hong_Kong/15_October_2010 15] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/16_October_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/17_October_2010&action=edit 17]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/18_October_2010&action=edit 18] [http://2010.igem.org/HKU-Hong_Kong/19_October_2010 19] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/20_October_2010&action=edit 20] [http://2010.igem.org/HKU-Hong_Kong/21_October_2010 21] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/22_October_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/23_October_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/24_October_2010&action=edit 24]
[http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/25_October_2010&action=edit 25] [http://2010.igem.org/HKU-Hong_Kong/26_October_2010 26] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=HKU-Hong_Kong/31_October_2010&action=edit 31]

Contents

Our official start:12 July 2010

After 2 weeks of training on basic techniques on biological lab work, we started our project by testing the functionality of the suicide gene.

Background

The biopart BBa_k112808 shipped is in the plasmid backbone pSB1A2. We tried to separate the biopart from the plasmid by enzyme digestion. After looking into the sequences, double enzyme digestion with NotI and XbaI was carried out.

Aim

To obtain biopart BBa_k112808 for further modifications and combination with other bioparts in different plasmid.

Results

12jul.jpg

A gel photo was taken for detection of cut DNA fragments.

BBa_k112808

Two bands are expected for the enzyme digestion on BBa_k112808. Yet, a band with ~4000bp was observed. This size matches with the SUM of the two fragments expected.

BBa_k112808:~1.8k bp

pSB1A2: ~1.9k bp

Some of the restriction sites might be absent for such a result to be obtained.

pET28a

The size of pET28a is 5369 bp, with reference to its genome map, the two fragemnts were expected to be ~5000bp and ~200bp. As a 1% agarose gel was used, one band at ~5000bp was expected. Consistence was observed in the digestion.

 

Retrieved from "http://2010.igem.org/HKU-Hong_Kong/12_July_2010"