Team:NYMU-Taipei

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<dt>Default display</dt>
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<dt>Your Promoter</dt>
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<span>Your Protein</span>
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<dt>Speedy mRNA Reporter</dt>
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<span>Speedy mRNA Reporter</span>
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<dt>Speedy Protein Reporter</dt>
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<span>Speedy Protein Reporter</span>
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<span>Speedy Switch</span>
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<span>Speedy Protein Degrader</span>
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<font size=5>'''SpeedyBac'''</font><br>
<font size=5>'''SpeedyBac'''</font><br>
<br>
<br>
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*'''<font size=3>Goal</font>:'''<br>
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*'''<font size=3>Goal</font>'''<br>
Provide a faster assay system for exploring the design rules of synthetic biology.
Provide a faster assay system for exploring the design rules of synthetic biology.
*'''<font size=3>Why do we want to do that?</font><br>
*'''<font size=3>Why do we want to do that?</font><br>
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There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing rapidly. Every year every igem teams will build one or more circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles connect mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules. <br>
+
There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing fast. Every year every igem teams will build genetic circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles for connecting mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not mostly through the expression of reporter genes. However, it would be much helpful if we could have information of quantitative description of gene expression in both space and time. For these reasons and for the future development of synthetic biology, we just have to speed up the experimental explorations of design rules. <br>
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*'''<font size=3>Specific aims:</font>'''<br>
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*'''<font size=3>Specific aims</font>'''<br>
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**Quantitative description of gene expression in both space and time.
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** detect gene expression quantitatively in both space and time.
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**Specific insight into the flow of genetic information.
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** specific insight into the flow of genetic information.
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**Speedy ways to report and stop gene expression.
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** provide speedy ways to report and stop gene expression.
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*'''<font size=3>Our design:</font>'''<br>
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----
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To achieve our specific aim, we have designed a novel reporting assay [[Team:NYMU-Taipei/Project/Speedy reporter|(Speedy reporter)]] for quickly detect and measure the mRNA location and quantity, it can be also use for protein detection. And we design a novel switch [[Team:NYMU-Taipei/Project/Speedy switch|(Speedy switch)]] for control the translation in gene expression. We have also designed a faster degradation system [[Team:NYMU-Taipei/Project/Speedy protein degrader |(Speedy protein degrader)]]; it allows us to regulate the degradation time for study the mRNA without the interference from translation and quickly stop the gene expression.<br>
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[[Image:Nymusyb2.png|500px]]
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'''The parts our project is made up of''':<br>
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* [[Team:NYMU-Taipei/Project/Speedy switch | Speedy switch]]
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** Faster production of protein by inducing the translation of pre-transcribed RNA molecules.
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* [[Team:NYMU-Taipei/Project/Speedy reporter| Speedy reporter]]
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** Using mRNA aptamers and split GFP-eIF4A reporter systems to show promoter activity faster.
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* [[Team:NYMU-Taipei/Project/Speedy protein degrader | Speedy protein degrader]]
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** Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins with LVA
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| style="vertical-align:top;" |
| style="vertical-align:top;" |
{{:Team:NYMU-Taipei/Our institute}}
{{:Team:NYMU-Taipei/Our institute}}
|}
|}
{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Latest revision as of 20:31, 27 October 2010

Default display
Your Promoter
Your Promoter
Your Protein
Your Protein
Speedy mRNA Reporter
Speedy mRNA Reporter
Speedy Protein Reporter
Speedy Protein Reporter
Speedy Switch
Speedy Switch
Speedy Protein Degrader
Speedy Protein Degrader

SpeedyBac

  • Goal

Provide a faster assay system for exploring the design rules of synthetic biology.

  • Why do we want to do that?

There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing fast. Every year every igem teams will build genetic circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles for connecting mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not mostly through the expression of reporter genes. However, it would be much helpful if we could have information of quantitative description of gene expression in both space and time. For these reasons and for the future development of synthetic biology, we just have to speed up the experimental explorations of design rules.

  • Specific aims
    • detect gene expression quantitatively in both space and time.
    • specific insight into the flow of genetic information.
    • provide speedy ways to report and stop gene expression.

Nymusyb2.png

  • Our design

To achieve our specific aim, we have designed a novel reporting device (Speedy reporter) for quickly detectin and measuring the mRNA location and quantity, it can also be used for protein detection. And we design a novel switch (Speedy switch) for control the mRNA translation of gene expression. We have also designed a faster degradation device (Speedy protein degrader); it allows us to regulate the degradation time for studying the mRNAs without the interference from translation and quickly stopping the gene expression.

Our SpeedyBac system is made up of the following three devices:

  • Speedy switch
    • Faster production of protein by inducing the translation of pre-existing mRNA molecules.
  • Speedy reporter
    • Using mRNA aptamers and split GFP-eIF4A reporter designs to detect promoter activity faster.
  • Speedy protein degrader
    • Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins tagged with LVA


The official web pages of our school - National Yang Ming University (NYMU):

  • [http://web.ym.edu.tw/front/bin/home.phtml in Chinese]
  • [http://nymu-e.web.ym.edu.tw/front/bin/home.phtml in English]

Click the following two links to see The Beauty of NYMU

  • [http://issue.ym.edu.tw/cia/new/ Take a panoramic scenery view of our university]
  • [http://issue.ym.edu.tw/cia/new/tw/ym720.html Take a tour of our university]

Retrieved from "http://2010.igem.org/Team:NYMU-Taipei"