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Team:UC Davis/notebook/onpathway.html
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<a name="construct"></a><p class="header"><b>Construct:</b></a><p> | <a name="construct"></a><p class="header"><b>Construct:</b></a><p> | ||
<img src="https://static.igem.org/mediawiki/2010/c/cf/Onpiecesx.jpg"><p> | <img src="https://static.igem.org/mediawiki/2010/c/cf/Onpiecesx.jpg"><p> | ||
| + | |||
<a name="stepone"></a><p class="header"><b>Step One</b></a><p> | <a name="stepone"></a><p class="header"><b>Step One</b></a><p> | ||
| - | <table border=0 margin="0" width=" | + | <table border=0 margin="0" width="600px" padding:0 margin:0> |
<tr> | <tr> | ||
<td><img src="https://static.igem.org/mediawiki/2010/d/dd/Rbsc0051x.jpg"></td> | <td><img src="https://static.igem.org/mediawiki/2010/d/dd/Rbsc0051x.jpg"></td> | ||
| Line 36: | Line 37: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td><img src="https://static.igem.org/mediawiki/2010/3/3f/Rbse1010x.jpg"></td> | + | <td width="350px"><img src="https://static.igem.org/mediawiki/2010/3/3f/Rbse1010x.jpg"></td> |
<td> | <td> | ||
</td> | </td> | ||
| Line 43: | Line 44: | ||
<a name="steptwo"></a><p class="header"><b>Step Two</b></a><p> | <a name="steptwo"></a><p class="header"><b>Step Two</b></a><p> | ||
| - | <table border=0 margin="0" width=" | + | <table border=0 margin="0" width="600px" padding:0 margin:0> |
| + | <tr> | ||
| + | <td width="350px"><img src="https://static.igem.org/mediawiki/2010/f/f8/Rbse1010f1610.jpg"></td> | ||
| + | <td> | ||
| + | <ul> | ||
| + | <li>RBS-RFP and F1610 are ligated together</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table><p><br /> | ||
| + | |||
| + | <a name="stepthree"></a><p class="header"><b>Step Three</b></a><p> | ||
| + | <table border=0 margin="0" width="600px" padding:0 margin:0> | ||
<tr> | <tr> | ||
| - | <td><img src="https://static.igem.org/mediawiki/2010/ | + | <td width="350px"><img src="https://static.igem.org/mediawiki/2010/2/2d/Rbsc0051rbse1010f1610.jpg"></td> |
<td> | <td> | ||
<ul> | <ul> | ||
| - | <li>RBS-C0051 and RBS-RFP are ligated together</li> | + | <li>RBS-C0051 and RBS-RFP-F1610 are ligated together</li> |
| - | <li>This step is where we have discovered bizarre transcription!!!</li> | + | <li><a href="https://2010.igem.org/Team:UC_Davis/notebook/c0051debug.html" class="help">This step is where we have discovered bizarre transcription!!!</a></li> |
</ul> | </ul> | ||
</td> | </td> | ||
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</table> | </table> | ||
| - | <a name=" | + | <a name="stepfour"></a><p class="header"><b>Step Four</b></a><p> |
| - | <table border=0 margin="0" width=" | + | <table border=0 margin="0" width="600px" padding:0 margin:0> |
<tr> | <tr> | ||
| - | <td><img src="https://static.igem.org/mediawiki/2010/2/2d/Rbsc0051rbse1010f1610.jpg"></td> | + | <td width="350px"><img src="https://static.igem.org/mediawiki/2010/2/2d/Rbsc0051rbse1010f1610.jpg"></td> |
| - | <td><ul><li>AFTER getting rid of the C0051 bar code, we have ligated the | + | <td><ul><li>AFTER getting rid of the C0051 bar code, we have ligated the MODIFIED c0051 to the RBS-RFP-F1610.</li></ul> |
</tr> | </tr> | ||
</table> | </table> | ||
| - | <a name=" | + | <a name="stepfive"></a><p class="header"><b>Step Five</b></a><p> |
| - | <table border=0 margin="0" width=" | + | <table border=0 margin="0" width="600px" padding:0 margin:0> |
<tr> | <tr> | ||
| - | <td><img src="https://static.igem.org/mediawiki/2010/8/8a/Onpiecescomplete.jpg"></td> | + | <td width="350px"><img src="https://static.igem.org/mediawiki/2010/8/8a/Onpiecescomplete.jpg"></td> |
<td><ul><li>After ligating the piece to the I12006 promoter, we have now completed the ON switch!</li></ul></td> | <td><ul><li>After ligating the piece to the I12006 promoter, we have now completed the ON switch!</li></ul></td> | ||
</tr> | </tr> | ||
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<li><a href="#stepone" class="help">Step One</a></li> | <li><a href="#stepone" class="help">Step One</a></li> | ||
<li><a href="#steptwo" class="help">Step Two</a></li> | <li><a href="#steptwo" class="help">Step Two</a></li> | ||
| + | <li><a href="#stepthree" class="help">Step Three</a></li> | ||
| + | <li><a href="#stepfour" class="help">Step Four</a></li> | ||
| + | <li><a href="#stepfive" class="help">Step Five</a></li> | ||
</ul> </td> | </ul> </td> | ||
</tr> | </tr> | ||
Latest revision as of 03:33, 28 October 2010
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