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Team:Queens-Canada/15 June 2010
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(New page: ==June 15, 2010== ====Liquid Culture==== Thai and Chris '''BBa_J45200''', '''BBa_J45120''' Products incubated in 37°C room on shaker. ====PCR==== Yuli '''BBa_I714891''' Program run u...) |
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| + | <h2>June 15, 2010</h2> | ||
| + | <h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3> | ||
Thai and Chris | Thai and Chris | ||
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Products incubated in 37°C room on shaker. | Products incubated in 37°C room on shaker. | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | |
Yuli | Yuli | ||
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Program run using TAQ with extension time of 30s and annealing temperature of 51°C. | Program run using TAQ with extension time of 30s and annealing temperature of 51°C. | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3> | |
Hao and Mary | Hao and Mary | ||
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Stored in -80 °C freezer. | Stored in -80 °C freezer. | ||
| - | + | <h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3> | |
Hao and Mary | Hao and Mary | ||
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Standard procedure followed. | Standard procedure followed. | ||
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| + | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 05:21, 27 October 2010
June 15, 2010
Liquid Culture
Thai and Chris
BBa_J45200, BBa_J45120
Products incubated in 37°C room on shaker.
PCR
Yuli
BBa_I714891
Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
Glycerol Stock
Hao and Mary
BBa_J45200, BBa_J45120
Change: Used 250 µL of 80% glycerol and 750 µL of overnight culture. Stored in -80 °C freezer.
Miniprep
Hao and Mary
BBa_J45200, BBa_J45120
Standard procedure followed.
