Team:MIT k415032
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<div class="bodybaby">K415031</div><br> | <div class="bodybaby">K415031</div><br> | ||
- | From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part: | + | From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415032"><b>See the Parts Registry Page!! →</b></a><br><br> |
<b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.<br><br> | <b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.<br><br> | ||
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<center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br> | <center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br> | ||
- | <a href="https://2010.igem.org/Team:MIT_k415031"><b>← K415031</b></a><a style="float:right;" href="https://2010.igem.org/Team: | + | <a href="https://2010.igem.org/Team:MIT_k415031"><b>← K415031</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415108"><b>K415108 →</b></a> |
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Latest revision as of 02:44, 27 October 2010
K415031 From the 2010 MIT iGEM Team.See the Parts Registry Page!! → Improved PLux/cI-OR Promoter This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. Slightly weaker binding than K415031. ← K415031K415108 → |