Latest revision as of 19:55, 27 October 2010

As summer project our experimental work began in August, after intensive brainstorming we finnaly decide

between two options.

*Inmunoresponse using Synthetic Biology.

*Cryobiology applied to plant crops.

After a selection process we chose the Cryobiology Project.

The final design of the expressions modules were as follow:

Next Notebook sheet is our reference for primers and ligations and for modules Assembly:

The AFP (Antifreeze Protein) was synthesized as follow:

Week #1

06th September - 10th September 2010

Monday

We got plasmid PSB1C3 from the Biobrick plate.

We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.

Some LB agar and 35ug/ml cloramphenicol plates made up .

We al ready made quimio and electro-competent cells for transformation and stored in aliquots in containers vials at -80 degrees..

Tuesday

We transformed DH5α cells with PSB1C3.

Wednesday

We have not obtained transformats cells.

We checked out Cloramphenicol stock concentration and attempt again the transformation used PSB1C3.

Thursday

The primers arrived and we proceed to amplify the modules by PCR.

The amplification was ok and we had looking for a nano spectrophotometer.

we made quantification of DNA concentrations

@@ Line 6: / Line 6: @@
 '''As summer project our experimental work began in August, after intensive brainstorming we finnaly decide
-'''between two options.'''
+'''between two options.
- *''Inmunoresponse using Synthetic Biology''
- *''Cryobiology applied to plant crops''
+=*'''Inmunoresponse using Synthetic Biology.'''=
-== '''After a selection process we chose the Cryobiology Project''' ==
+=*'''Cryobiology applied to plant crops.'''=
+== '''After a selection process we chose the Cryobiology Project.''' ==
 '''The final design of the expressions modules were as follow:'''
@@ Line 34: / Line 37: @@
 ==''Monday''==
-==='''We discussed and concluded the Igem’s vector (vial with green cover) is not enough.'''===
-==='''First step transform only vector to get enough and begin ligations.'''===
-==='''We are going to  transform Psb1C3  from plate.  For this:'''===
-*'''Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.'''
+*''' We got plasmid PSB1C3 from the Biobrick plate. '''
-*''' 5 LB agar and 35ug/ml cloramphenicol plates made up . '''
+*''' We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.'''
-*'''Complete procedure for making quimio and electro-competent cells.'''
+*''' Some LB agar and 35ug/ml cloramphenicol plates made up . '''
+*''' We al ready made quimio and electro-competent cells for transformation and stored in aliquots in containers vials at -80 degrees..'''
 ==''Tuesday''==
-==='''Completed competent cells and stored aliquots at -80 degrees each vial with 150μl.'''===
-==='''We transformed DH5α cells with Psb1C3.'''===
+==='''We transformed DH5α cells with PSB1C3.'''===
 ==''Wednesday''==
-==='''For a strange reason we have not transformats cells'''===
+'''We have not obtained  transformats cells.'''
-==='''today we are going to check out the Cloramphenicol'''===
-==='''dose and try again the transformation with Psb1C3.'''===
+'''We checked out Cloramphenicol stock concentration and attempt again the transformation used PSB1C3.'''
 ==''Thursday''==
-==='''In vector’s absence we have recived the primer’s synthesis'''===
+'''The primers arrived and we proceed to amplify the modules by PCR.'''
-==='''and proceed to amplify by PCR.'''===
 [[Image:PCR.gif]]
-==='''The amplification is correct and we have to looking for a nanodrop'''===
+'''The amplification was ok and we had looking for a nano spectrophotometer.'''
-==='''to quantify DNA’s concentrations.'''===
+'''we made quantification of DNA concentrations'''

Team:Mexico-UNAM-CINVESTAV/Notebook/Week One

From 2010.igem.org