Team:Stockholm/Results/BioBricks

From 2010.igem.org

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{{Stockholm/Results}}
{{Stockholm/Results}}
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==BioBricks==
===''Trans''-Activating Transcriptional Activator (TAT)===
===''Trans''-Activating Transcriptional Activator (TAT)===
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:'''Part name:''' TAT cell-penetrating peptide<br />
:'''Part name:''' TAT cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
-
:'''Sequenced:''' <span style="color:green;">Yes</span> (fasta)<br />
+
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp1 premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
-
<br /><br />
+
<br />
====[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part====
====[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part====
Line 19: Line 21:
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
:'''Part name:''' N-part TAT cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#TAT_cell_penetrating_peptide_.28TAT.29|more]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_12_premix.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_12_premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===Low Molecular Weight Protamine (LMWP)===
===Low Molecular Weight Protamine (LMWP)===
Line 31: Line 35:
:'''Part name:''' LMWP cell-penetrating peptide<br />
:'''Part name:''' LMWP cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
-
:'''Sequenced:''' <span style="color:green;">Yes</span> (fasta)<br />
+
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:Cpp2 premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
====[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part====
====[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part====
Line 39: Line 44:
:'''Part name:''' N-part LMWP cell-penetrating peptide<br />
:'''Part name:''' N-part LMWP cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Low_molecular_weight_protamine_.28LMWP.29|more]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_11_premix.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_11_premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===Transportan 10 (Tp10)===
===Transportan 10 (Tp10)===
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:'''Part name:''' Tp10 cell-penetrating peptide<br />
:'''Part name:''' Tp10 cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
-
:'''Sequenced:''' <span style="color:green;">Yes</span> (fasta)<br />
+
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Cpp12 premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
====[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part====
====[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part====
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:'''Part name:''' N-part Tp10 cell-penetrating peptide<br />
:'''Part name:''' N-part Tp10 cell-penetrating peptide<br />
-
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Cell-penetrating peptide ([[Team:Stockholm/Project_Idea/Proteins#Transportan_10_.28Tp10.29|more]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_5_premix.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.nCCP_5_premix.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)===
===[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)===
Line 69: Line 79:
:'''Part name:''' Human basic fibroblast growth factor, bFGF<br />
:'''Part name:''' Human basic fibroblast growth factor, bFGF<br />
-
:'''Property:''' Growth factor ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Growth factor ([[Team:Stockholm/Project_Idea/Proteins#Human_basic_fibroblast_growth_factor_.28bFGF.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt341 C &rarr; T
 +
:'''Removed restr. site(s):''' AgeI
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.bFGF_VR_premix_fasta.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.bFGF_VR_premix_fasta.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)===
===[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)===
Line 79: Line 93:
:'''Part name:''' Superoxide dismutase 1 protein<br />
:'''Part name:''' Superoxide dismutase 1 protein<br />
-
:'''Property:''' Catalyzes the reaction O<sup>-</sup><sub>2</sub> + O<sup>-</sup><sub>2</sub> + 2H<sup>+</sup> → H<sub>2</sub>O<sub>2</sub> + O<sub>2</sub> ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Catalyzes the reaction O<sup>-</sup><sub>2</sub> + O<sup>-</sup><sub>2</sub> + 2H<sup>+</sup> → H<sub>2</sub>O<sub>2</sub> + O<sub>2</sub> ([[Team:Stockholm/Project_Idea/Proteins#Superoxide_dismutase_1_.28SOD1.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt331 A &rarr; G
 +
:'''Removed restr. site(s):''' PfeI
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.SOD_VR_premix_fasta.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.SOD_VR_premix_fasta.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)===
===[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)===
Line 89: Line 107:
:'''Part name:''' Yeast copper chaperone protein<br />
:'''Part name:''' Yeast copper chaperone protein<br />
-
:'''Property:''' Mediates the delivery of copper ions to SOD1 ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Mediates the delivery of copper ions to SOD1 ([[Team:Stockholm/Project_Idea/Proteins#Yeast_copper_chaperon_.28yCCS.29|more]])<br />
 +
:'''Edited nucleotide(s):''' nt257 T &rarr; C
 +
:'''Removed restr. site(s):''' EcoRI
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.yCCS_VR_premix_fasta.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.yCCS_VR_premix_fasta.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)===
===[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)===
[[image:ProteinA_BioBrick.png]]
[[image:ProteinA_BioBrick.png]]
-
:'''Part name:''' protein A<br />
+
:'''Part name:''' Protein A Z-domain<br />
-
:'''Property:''' - ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Immunoglobulin Fc region binding ([[Team:Stockholm/Project_Idea/Proteins#Protein_A.2C_z_domain|more]])<br />
 +
:'''Edited nucleotide(s):''' -
 +
:'''Removed restr. site(s):''' -
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.ProtA VR premix fasta.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.ProtA VR premix fasta.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
 +
<br />
----
----
 +
<br />
===[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)===
===[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)===
[[image:IdeS_BioBrick.png]]
[[image:IdeS_BioBrick.png]]
-
:'''Part name:''' igg protease<br />
+
:'''Part name:''' Immunoglobulin G protease (IdeS)<br />
-
:'''Property:''' - ([[Team:Stockholm/Project Idea/Proteins|more]])<br />
+
:'''Property:''' Immunoglobulin G protease ([[Team:Stockholm/Project_Idea/Proteins#IgG_protease_.28IdeS.29|more]])<br />
 +
:'''Edited nucleotide(s):''' -
 +
:'''Removed restr. site(s):''' -
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.IgGp_VR_premix_fasta.txt|fasta]])<br />
:'''Sequenced:''' <span style="color:green;">Yes</span> ([[Media:PSB1C3.IgGp_VR_premix_fasta.txt|fasta]])<br />
:'''Works:''' <span style="color:red;">Not known</span>
:'''Works:''' <span style="color:red;">Not known</span>
-
 
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|}
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{{Stockholm/Footer}}
{{Stockholm/Footer}}

Latest revision as of 01:03, 28 October 2010


Contents

BioBricks

Trans-Activating Transcriptional Activator (TAT)

[http://partsregistry.org/Part:BBa_K380000 BBa_K380000]: Standard part

TAT BioBrick.png

Part name: TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380001 BBa_K380001]: N-part

NTAT BioBrick.png

Part name: N-part TAT cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known




Low Molecular Weight Protamine (LMWP)

[http://partsregistry.org/Part:BBa_K380002 BBa_K380002]: Standard part)

LMWP BioBrick.png

Part name: LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380003 BBa_K380003]: N-part

NLMWP BioBrick.png

Part name: N-part LMWP cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known




Transportan 10 (Tp10)

[http://partsregistry.org/Part:BBa_K380004 BBa_K380004]: Standard part

Tp10 BioBrick.png

Part name: Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known


[http://partsregistry.org/Part:BBa_K380005 BBa_K380005]: N-part

NTp10 BioBrick.png

Part name: N-part Tp10 cell-penetrating peptide
Property: Cell-penetrating peptide (more)
Sequenced: Yes (fasta)
Works: Not known




[http://partsregistry.org/Part:BBa_K380006 BBa_K380006]: Human Basic Fibroblast Growth Factor (bFGF)

BFGF BioBrick.png

Part name: Human basic fibroblast growth factor, bFGF
Property: Growth factor (more)
Edited nucleotide(s): nt341 C → T
Removed restr. site(s): AgeI
Sequenced: Yes (fasta)
Works: Not known




[http://partsregistry.org/Part:BBa_K380007 BBa_K380007]: Superoxide dismutase 1 (SOD1)

SOD1 BioBrick.png

Part name: Superoxide dismutase 1 protein
Property: Catalyzes the reaction O-2 + O-2 + 2H+ → H2O2 + O2 (more)
Edited nucleotide(s): nt331 A → G
Removed restr. site(s): PfeI
Sequenced: Yes (fasta)
Works: Not known




[http://partsregistry.org/Part:BBa_K380008 BBa_K380008]: Yeast Copper Chaperone (yCCS)

YCCS BioBrick.png

Part name: Yeast copper chaperone protein
Property: Mediates the delivery of copper ions to SOD1 (more)
Edited nucleotide(s): nt257 T → C
Removed restr. site(s): EcoRI
Sequenced: Yes (fasta)
Works: Not known




[http://partsregistry.org/Part:BBa_K380009 BBa_K380009]: Protein A Z-domain (PtA-Z)

ProteinA BioBrick.png

Part name: Protein A Z-domain
Property: Immunoglobulin Fc region binding (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known




[http://partsregistry.org/Part:BBa_K380010 BBa_K380010]: Immunoglobulin G protease (IdeS)

IdeS BioBrick.png

Part name: Immunoglobulin G protease (IdeS)
Property: Immunoglobulin G protease (more)
Edited nucleotide(s): -
Removed restr. site(s): -
Sequenced: Yes (fasta)
Works: Not known





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/