Team:UNIPV-Pavia/Calendar/October
From 2010.igem.org
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- | <td colspan=" | + | <td colspan="3"> {{UNIPV-Pavia/header}} </td> |
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+ | |||
+ | <table class="menu" border="0" width="100%"> | ||
+ | <tr> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/October/settimana1|Week 1]] | ||
+ | </td> | ||
+ | <td align="center" style="padding:0; height:20px"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/October/settimana2|Week 2]] | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/October/settimana3|Week 3]] | ||
+ | </td> | ||
+ | <td align="center"> | ||
+ | [[Team:UNIPV-Pavia/Calendar/October/settimana4|Week 4]] | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
+ | |||
<html><p align="center"><font size="4"><b>OCTOBER</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>OCTOBER</b></font></p></html><hr><br> | ||
+ | Bio-Lab activity proceeds!<br><br> | ||
+ | <font size = "4"><b><u>Select the day you are interested in to find out the details!</u></b></font><br><br> | ||
<table class=cont border="0" width="30%" align="center" valign="top"> | <table class=cont border="0" width="30%" align="center" valign="top"> | ||
<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | ||
- | <tr><td align="center" valign="top">1st</td><td align="left"> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left"> |
+ | 99° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73). | ||
+ | Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C. | ||
- | + | Miniprep and Nanodrop quantification for: I31, I35, I60 (they were also sent sequencing), INTEIN, pSB1C3-1, pSB1C3-2, pSB1C3-3 and I37 (they were no sent sequencing). | |
+ | Digest and gel run/cut/extraction/quantification of these samples were performed. | ||
- | <td | + | ON ligation of: I74: I31 + I35 and INTEIN_C3: INTEIN + pSB1C3. |
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left"> | ||
+ | 100° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm. | ||
+ | Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run. | ||
+ | |||
+ | Transformation of I74 and INTEIN_C3 (1ul) in E. coli TOP10. They were plated on LB+Amp100 and LB+Cm34 agar plates respectively. They were let grow ON at +37°C. | ||
+ | |||
+ | Inoculum of I55-1 into 5 ml LB+Amp. ON growth at +37°C, 220 rpm. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left"> | ||
+ | 101° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5. | ||
+ | |||
+ | Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm. | ||
+ | |||
+ | Miniprep and quantification of:I55-1, I55-1bis. I55-1bis was digested E-S for about three hours and than gel run/cut. | ||
+ | |||
+ | Colony PCR for I74-1..6. Gel run for PCR samples and I55 (E-S) and cut for this one. | ||
+ | |||
+ | Gel extraction and quantification of I55. | ||
+ | |||
+ | Ligation of I55 with already available DNA: I75: I55 + I37 and I77: I55 + I57. | ||
+ | |||
+ | Inoculum of I35 (last quantification after gel extraction was too poor; we will digest it again E-P) and I54 into 5 ml LB+Amp: ON growth at +37°C, 220 rpm. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left"> | ||
+ | 102° Bio-Lab - Miniprep and quantification of: INTEIN_C3, I74-8, I54, I35. Digest and gel run/cut/quantification of these samples. | ||
+ | |||
+ | Ligations of I78=I74+INTEIN_C3. | ||
+ | |||
+ | Transormation (1ul) into E. coli TOP10 of:I75, I77. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
+ | |||
+ | <b>Today we start to transfer all parts that will be sent in <partinfo>pSB1C3</partinfo> plasmid.</b> | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | ||
+ | 103° Bio-Lab - Digestions were performed again for I55, I37, I57, I31 and I54. Digestions were let at +37°C ON. | ||
+ | |||
+ | Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | ||
+ | |||
+ | ON ligation of I79: I60 + INTEIN_C3. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | ||
+ | 104° Bio-Lab - E. coli TOP10 plated on LB+Amp agar plate showed red colonies!!! | ||
+ | |||
+ | Transformation of available ligations I75 and I77 (1ul) into E. coli TOP10. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
+ | |||
+ | Gel run and cut for ON digestions and Nanodrop quantification of I54 and I55. Ligation of I76=I55+I54. | ||
+ | |||
+ | PCR from colony screening for I78. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | ||
+ | 105° Bio-Lab - I78 screening gel run. Glycerol stock and miniprep for I78-3. | ||
+ | |||
+ | Colony PCR for screening of I79 (we pick eleven colonies). | ||
+ | |||
+ | Transformation (1ul) of I0_1C3, I3_1C3, I7_1C3, I8_1C3, I9_1C3, I12_1C3 and I76 into E. coli DH5-alpha. | ||
+ | |||
+ | They were plated on LB+Cm34 agar plates (except for I76 that was plated on LB+Amp agar plate) and let grow ON at +37°C. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | ||
+ | 106° Bio-Lab - Glycerol stock, miniprep and Nanodrop quantification of I79-2.This DNA was sent to BMR Genomics for sequencing. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | ||
+ | 107° Bio-Lab -A new part was cloned with these ones, named I80. It is the basic part to build self-inducible promoters. | ||
+ | |||
+ | <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> | ||
+ | 108° Bio-Lab - Inoculum into 4 ml LB+Amp of: I75-1/2/3, I76-1/2, I77-1/2/3, I47, I48, I49. They were let grow at +37°C, 220 rpm. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left"> | ||
+ | 109° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>.<br>Inoculum of MC42-A, MC43-A, MG42-B, MG43-A, MC43_flip-a,b,c, MG43_flip-a,b,c, MG1655.<br> | ||
+ | 15° Meeting - Updating about Bio-Lab activity. Decisions about poster and presentation. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left"> | ||
+ | 110° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. <br>Screening PCR. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left"> | ||
+ | 111° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left"> | ||
+ | 112° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left"> | ||
+ | 113° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left"> | ||
+ | 114° Bio-Lab - <b>We are now moving all our parts to pSB1C3 plasmid</b>. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left"> | ||
+ | 115° Bio-Lab - We have finally completed the parts transfer to pSB1C3 plasmid. We sent the parts to MIT. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left"> | ||
+ | 116° Bio-Lab - Wiki update and definition of results. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left"> | ||
+ | 117° Bio-Lab - Wiki update and definition of results. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left"> | ||
+ | 118° Bio-Lab - Wiki update and definition of results. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left"> | ||
+ | 119° Bio-Lab - Wiki update and definition of results. | ||
+ | <br>16°Meeting - Updating about biolab activity and definition of last details. | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left"> | ||
+ | 120° Bio-Lab - Wiki update and definition of results. | ||
+ | </td></tr> | ||
+ | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left"> | ||
+ | 121° Bio-Lab - Wiki update and definition of results | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left"> | ||
+ | 122° Bio-Lab - Wiki update and definition of results | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left"> | ||
+ | 123° Bio-Lab - Wiki update and definition of results | ||
+ | </td></tr> | ||
+ | |||
+ | </table> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <td width="15%" valign="top"> | ||
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Latest revision as of 18:18, 27 October 2010
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