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Team:Stockholm/23 June 2010
From 2010.igem.org
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'' '''Note:''' The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.'' | '' '''Note:''' The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.'' | ||
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| + | ===Plasmid preparation of pEX vector=== | ||
| + | Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm. | ||
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| + | ===Transformation with pMA vector=== | ||
| + | The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3). | ||
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| + | * Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates. | ||
=Hassan= | =Hassan= | ||
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#Molecular Maya | #Molecular Maya | ||
#Chimera | #Chimera | ||
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| + | {{Stockholm/Footer}} | ||
Latest revision as of 10:31, 26 October 2010
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Contents |
Andreas
Testing Top10 competent cells and antibiotic agar plates
Results from 22/6
| Transformed plasmid | Plate | Growth |
| pEX | 100 ug/ml Amp | Yes |
| None | 100 ug/ml Amp | No |
| pEX | 25 ug/ml Cm | No |
| None | 25 ug/ml Cm | No |
Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.
Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.
Plasmid preparation of pEX vector
Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.
Transformation with pMA vector
The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).
- Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.
Hassan
Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.
softwares used:
- Molecular Maya
- Chimera
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