Team:Stockholm/23 June 2010

From 2010.igem.org

(Difference between revisions)
m
 
(4 intermediate revisions not shown)
Line 3: Line 3:
=Andreas=
=Andreas=
-
===Test===
+
===Testing Top10 competent cells and antibiotic agar plates===
 +
 
 +
''Results from 22/6''
 +
 
 +
{| border="1" cellspacing="0" cellpadding="2"
 +
|'''Transformed plasmid'''
 +
|'''Plate'''
 +
|'''Growth'''
 +
|-
 +
|pEX
 +
|100 ug/ml Amp
 +
|Yes
 +
|-
 +
|None
 +
|100 ug/ml Amp
 +
|No
 +
|-
 +
|pEX
 +
|25 ug/ml Cm
 +
|No
 +
|-
 +
|None
 +
|25 ug/ml Cm
 +
|No
 +
|}
 +
 
 +
Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.
 +
 
 +
'' '''Note:''' The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.''
 +
 
 +
===Plasmid preparation of pEX vector===
 +
Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.
 +
 
 +
===Transformation with pMA vector===
 +
The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).
 +
 
 +
* Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.
=Hassan=
=Hassan=
Line 13: Line 49:
#Molecular Maya
#Molecular Maya
#Chimera
#Chimera
 +
 +
{{Stockholm/Footer}}

Latest revision as of 10:31, 26 October 2010

Home Project Idea Lab Work Results Modelling Team       Follow us on and        2010.igem.org

↳   Notebook Safety Protocols


Contents

Andreas

Testing Top10 competent cells and antibiotic agar plates

Results from 22/6

Transformed plasmid Plate Growth
pEX 100 ug/ml Amp Yes
None 100 ug/ml Amp No
pEX 25 ug/ml Cm No
None 25 ug/ml Cm No

Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.

Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.

Plasmid preparation of pEX vector

Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.

Transformation with pMA vector

The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).

  • Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.

Hassan

Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.

softwares used:

  1. Molecular Maya
  2. Chimera





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/23_June_2010"