"

From 2010.igem.org

Revision as of 11:11, 26 October 2010 (view source) Hassanforoughiasl (Talk | contribs) ← Older edit		Latest revision as of 14:52, 26 October 2010 (view source) Mimmi (Talk | contribs) (→Mimmi)
(One intermediate revision not shown)
Line 43:		Line 43:
	==== Gel ====		==== Gel ====
-	[[Image:Place_for_picture.jpg|200px|thumb|left|]]	+	[[Image:2010-10-17_pEX.SOD_operon.jpg|250px|thumb|left|]]
	{|		{|
	! well		! well
Line 89:		Line 89:
	| pC.Sh_yC 4		| pC.Sh_yC 4
	|}		|}
		+

---

Latest revision as of 14:52, 26 October 2010

Contents 1 Mimmi 1.1 clone non-CPP operon 1.1.1 colony PCR 1.1.2 Gel 1.1.3 new colony PCR 1.1.4 ON cultures

Mimmi

clone non-CPP operon

colony PCR

mix		x12		primers		samples
mastermix	24.5			pEX_VF		pEX.SOD.RBS.yCCS 1-4
DNA	0.5			pEX_VR		pEX.SOD.his.RBS.yCCS 1-4
tot	25µl					pEX.his.SOD.RBS.yCCS 1-4
						positive control (pSB1C3.LMWP.SOD.his)

Gel

well	sample	well	sample
1	ladder	12	ladder
2	pEX.S_yC 1	13	pEX.hS_yC 1
3	pEX.S_yC 2	14	pEX.hS_yC 2
4	pEX.S_yC 3	15	pEX.hS_yC 3
5	pEX.S_yC 4	16	pEX.hS_yC 4
6	pEX.Sh_yC 1
7	pC.Sh_yC 2
8	pC.Sh_yC 3
9	pC.Sh_yC 4

new colony PCR

mix		x7		primers		samples
mastermix	24.5			pEX_VF		pEX.SOD.his.RBS.yCCS 1-7
DNA	0.5			pEX_VR
tot	25µl

ON cultures

• pEX.SOD.RBS.yCCS 4
• pEX.his.SOD.RBS.yCCS 3
• pEX.SOD
• pEX.yCCS