Team:Groningen/Biobricks

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'''Summary:'''<br>
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'''Summary:'''
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Below is given a short description of all the biobricks in our registry.<br><br>
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'''Chaplins'''<br><br>
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Below is given a short description of all the biobricks in our registry.
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
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<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive<br>
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'''Chaplins'''
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. <br>
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.    <br>
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE) <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
+
<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive
-
<partinfo>BBa_K305005</partinfo> Sortase srtA <br>
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Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall<br><br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
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'''Promotors'''<br><br>
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<partinfo>BBa_K305006</partinfo> yqxMpr <br>
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
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yqxM promotor effective in biofilm formation, regulates tasA production<br>
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<partinfo>BBa_K305007</partinfo> srfApr 1<br>
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)  
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Promotor for the surfactin regulatory gene.<br>
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<partinfo>BBa_K305008</partinfo> srfApr 2<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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Promotor for the surfactin regulatory gene.<br>
+
 
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<partinfo>BBa_K305009</partinfo> srfApr1>GFP  <br>
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<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)
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Promotor for the surfactin regulatory gene with GFP. <br>
+
 
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<partinfo>BBa_K305010</partinfo> srfApr2>GFP<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
-
Promotor for the surfactin regulatory gene with GFP. <br><br>
+
 
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'''Plasmid Backbone'''<br><br>
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<partinfo>BBa_K305005</partinfo> Sortase srtA  
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<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis<br>
+
 
 +
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
 +
 
 +
 
 +
 
 +
'''Promotors'''
 +
 
 +
<partinfo>BBa_K305006</partinfo> yqxMpr  
 +
 
 +
yqxM promotor effective in biofilm formation, regulates tasA production
 +
 
 +
<partinfo>BBa_K305007</partinfo> srfApr 1
 +
 
 +
Promotor for the surfactin regulatory gene.
 +
 
 +
<partinfo>BBa_K305008</partinfo> srfApr 2
 +
 
 +
Promotor for the surfactin regulatory gene.
 +
 
 +
<partinfo>BBa_K305009</partinfo> srfApr1>GFP   
 +
 
 +
Promotor for the surfactin regulatory gene with GFP.
 +
 
 +
<partinfo>BBa_K305010</partinfo> srfApr2>GFP
 +
 
 +
Promotor for the surfactin regulatory gene with GFP.
 +
 
 +
 
 +
 
 +
'''Plasmid Backbone'''
 +
 
 +
<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis
 +
 
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
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Latest revision as of 12:01, 26 October 2010

Biobricks

Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>


Summary:

Below is given a short description of all the biobricks in our registry.


Chaplins

<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

<partinfo>BBa_K305005</partinfo> Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall


Promotors

<partinfo>BBa_K305006</partinfo> yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

<partinfo>BBa_K305007</partinfo> srfApr 1

Promotor for the surfactin regulatory gene.

<partinfo>BBa_K305008</partinfo> srfApr 2

Promotor for the surfactin regulatory gene.

<partinfo>BBa_K305009</partinfo> srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

<partinfo>BBa_K305010</partinfo> srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.


Plasmid Backbone

<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.






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Retrieved from "http://2010.igem.org/Team:Groningen/Biobricks"