Team:TU Delft/18 June 2010 content

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We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
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=Cleaning=
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After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.  
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We also booked a hotel in Boston!
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We also booked a hotel in Boston!  
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===Lab work===
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=Lab work=
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Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:
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==Emulsifier==
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Pieter worked on the emulsifying activity assay. Four samples were tested:  
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1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|Sample
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|'''#'''
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|Amount of SDS (%)
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|'''Amount of SDS (%)'''
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|Hexalkane (mL)
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|'''Hexalkane (mL)'''
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|-
|1
|1
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|Blank
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|0
|0
|0
|-
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|-
|4
|4
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|1%
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|1
|0.02
|0.02
|}
|}
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Tris buffer (pH 8) was added to a total volume of 7.5 ml.
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2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.  
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Incubate 1 hour at 30 °C at shaken at 200 RPM.
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3. Shake 1 hour with 200 strokes/min at 30°C
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Absorbance measured at 600 nm.
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4. Measure absorbance OD600
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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|Sample
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|'''#'''
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|A600
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|'''A600'''
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|-
|1
|1
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|0.2
|0.2
|}
|}
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==Biobricks==
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By Hugo
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I've got some colonies in the plates, however there's contamination with some yellow colonies :s
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Probably, we will have to prepare a new stock of competent cells.

Latest revision as of 08:28, 26 August 2010

Contents

Cleaning

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Emulsifier

Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

# Amount of SDS (%) Hexalkane (mL)
1 0 0
2 0 0.02
3 0.01 0.02
4 1 0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

# A600
1 0
2 0
3 0.4
4 0.2

Biobricks

By Hugo

I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.

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