Team:Michigan/Quorum Sensing
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=Quorum Sensing Team= | =Quorum Sensing Team= | ||
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**AI-2 reporter & LuxS null mutant (''E. coli'', does not produce AI-2, amp and kan-resistant) | **AI-2 reporter & LuxS null mutant (''E. coli'', does not produce AI-2, amp and kan-resistant) | ||
Removed these strains from 4°C -- they are stored in soft agar stab cultures | Removed these strains from 4°C -- they are stored in soft agar stab cultures | ||
+ | |||
Made one streak plate on LB+amp for each strain from stabs | Made one streak plate on LB+amp for each strain from stabs | ||
- | *placed in | + | *placed in 37°C (rm. 1239) |
Made one 2mL LB+amp broth cultures in a 15mL tube for each strain from stabs | Made one 2mL LB+amp broth cultures in a 15mL tube for each strain from stabs | ||
*placed in 30°C, 200 rpm shaking (rm. 1230) | *placed in 30°C, 200 rpm shaking (rm. 1230) | ||
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*stored in iGEM box -- -80°C Lin Lab | *stored in iGEM box -- -80°C Lin Lab | ||
Plated LuxS<sup>-</sup> and MarC<sup>-</sup> mutants and ''Pseudomonas'' strains on LB | Plated LuxS<sup>-</sup> and MarC<sup>-</sup> mutants and ''Pseudomonas'' strains on LB | ||
+ | |||
Plated W3110 and MDAI2 on LB+amp+kan | Plated W3110 and MDAI2 on LB+amp+kan | ||
*placed all six plates in 37°C ERB 1239 | *placed all six plates in 37°C ERB 1239 | ||
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**W3110: '''204.86''' | **W3110: '''204.86''' | ||
**MDAI2: '''198.34''' | **MDAI2: '''198.34''' | ||
- | ''OK, | + | ''OK, I don't know if these have GFP or what. At any rate, if they do, the MDAI2 is too close to W3110. We'll probably have to work with the YFP biobrick instead.'' |
Got ''C. vulgaris'' from Bobby Levine | Got ''C. vulgaris'' from Bobby Levine | ||
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Autoclaved waste flasks | Autoclaved waste flasks | ||
*55 min (30 sterilize) | *55 min (30 sterilize) | ||
- | * | + | *25°C |
- | + | ||
== 8/3/2010 == | == 8/3/2010 == |
Latest revision as of 01:14, 27 October 2010