"

From 2010.igem.org

Revision as of 04:22, 26 October 2010 (view source) Jesús (Talk | contribs) ← Older edit		Latest revision as of 03:45, 27 October 2010 (view source) Jesús (Talk | contribs)
(9 intermediate revisions not shown)
Line 11:		Line 11:
	==='''Planned and prepared for tomorrow's electroporation'''===		==='''Planned and prepared for tomorrow's electroporation'''===
-	*'''Depart electroporator.'''	+	*'''Electroporator departed.'''
-	*'''Made up LB agar plates and cloramphenicol 100μl.'''	+	*'''Made up LB agar plates with cloramphenicol 35ng/ul.'''
-	*'''Plan to transform Psb1C3 froma plate.'''	+	*'''We  planned how to transform Psb1C3 from plate.'''
-	==='''We were looking for a nanodrop to quantify DNA’s concentrations'''===	+	==='''We still looking for a nano spectrophotometer to quantify DNA’s concentrations'''===
-	==='''and tried to make ligations Psb1C3 with each one of primers'''===	+	==='''and tried to make ligations Psb1C3 with each PCR product'''===
-	==='''(our this year's bricks).'''===	+	==='''( current  biobricks).'''===
	==''Tuesday''==		==''Tuesday''==
-	==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight.'''===	+	==='''Transformed DH5α competent cells, and kept 10 plates incubating 37ºC overnight.'''===
		+	*'''Prepared solutions for miniprep via Alkalin lysis method.'''
-	==''Wednsday''==
-	==='''Results previous day transformations only two plates were completed unsucessful.'''===	+	==''Wednesday''==
-	*'''Prepared solutions for miniprep via Alcalin lisis method'''	+	==='''Previous day transformations showed only two plates were sucessfully.'''===
	[[Image:Imagen2.gif]]		[[Image:Imagen2.gif]]
		+
		+
		+	*'''We kept the inoculated cells on  LB liquid medium at 37ºC overnight, four falcon tubes each containing  30ml.'''
	==''Thursday''==		==''Thursday''==
-	==='''The inoculum for miniprep we prepared 6 vector’s vials'''===	+	==='''We did the miniprep however we do not get positive results'''===
-	==='''without positive results. Maybe the solutions for alcaline'''===	+	===''' It could be that the solutions for alkalin lysis are not working.'''===
-	==='''lisis are not well.'''===	+
	[[Image:Imagen3.gif|500px]]		[[Image:Imagen3.gif|500px]]
-	*'''We have discused over how to quantify our cold sistem response'''	+	*'''We have discussed about the quantification of our cold sistem response'''
-	'''we gonig to use a promoter sistem, the funcional casset  using J13002  as follow'''	+	'''we are going to use a promoter system, the functional cassette with J13002  as follow:'''
-	*'''Promoter + our functional secuence + GFP'''	+	*'''Promoter + our functional sequence + GFP (green fluorescent protein)'''
Line 50:		Line 52:
-	*'''Promoter + our functional secuence + AFP (Anty freeze protein)	+	*'''Promoter + our functional sequence + AFP (anti freeze protein)
	[[Image:Imagen4.gif]]		[[Image:Imagen4.gif]]
-	*'''We have had a meeting with our Genomics frend’s we going to get a'''	+	=='''We had a meeting with our Genomic's friends and we are going to get a'''==
-	'''colaboration on Modeling and probably at wet lab too.'''	+	=='''collaboration on Modeling and maybe also at wet lab.'''==

---

Latest revision as of 03:45, 27 October 2010

Week #3

20th September -24th September 2010

Monday

Planned and prepared for tomorrow's electroporation

• Electroporator departed.
• Made up LB agar plates with cloramphenicol 35ng/ul.
• We planned how to transform Psb1C3 from plate.

We still looking for a nano spectrophotometer to quantify DNA’s concentrations

and tried to make ligations Psb1C3 with each PCR product

( current biobricks).

Tuesday

Transformed DH5α competent cells, and kept 10 plates incubating 37ºC overnight.

• Prepared solutions for miniprep via Alkalin lysis method.

Wednesday

Previous day transformations showed only two plates were sucessfully.

• We kept the inoculated cells on LB liquid medium at 37ºC overnight, four falcon tubes each containing 30ml.

Thursday

We did the miniprep however we do not get positive results

It could be that the solutions for alkalin lysis are not working.

• We have discussed about the quantification of our cold sistem response

we are going to use a promoter system, the functional cassette with J13002 as follow:

• Promoter + our functional sequence + GFP (green fluorescent protein)

• Promoter + our functional sequence + AFP (anti freeze protein)

We had a meeting with our Genomic's friends and we are going to get a

collaboration on Modeling and maybe also at wet lab.