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Week of 10/4
From 2010.igem.org
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-->J23108+LuxR (B) | -->J23108+LuxR (B) | ||
-->J23116+LuxR (B) | -->J23116+LuxR (B) | ||
| - | |||
==October 6th== | ==October 6th== | ||
| + | |||
| + | |||
| + | We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample. | ||
| + | |||
*Colony PCR: | *Colony PCR: | ||
| Line 24: | Line 27: | ||
6. R0063+RFP + J23116+LuxR | 6. R0063+RFP + J23116+LuxR | ||
| - | They were then run on a gel after being | + | They were then run on a gel after being PCR'd. |
*Gel Order: | *Gel Order: | ||
| + | - 1.a,b,c | ||
| + | - 2.a,b,c | ||
| + | - 3.a,b,c | ||
| + | - 4.a,b,c | ||
| + | - 5.a,b,c | ||
| + | - 6.a,b,c | ||
| + | |||
| + | <center>[[Image:10-06-10.jpg]]</center> | ||
| + | |||
| + | *Grown In Flasks: | ||
| + | -1a (R1062+RFP+J23100+LuxR) | ||
| + | -2a (R1062+RFP+J23108+LuxR) | ||
| + | -3a (R1062+RFP+J23116+LuxR) | ||
| + | -4a (R0063+RFP+J23100+LuxR) | ||
| + | -5a (R0063+RFP+J23108+LuxR) | ||
| + | -6a (R0063+RFP+J23116+LuxR) | ||
| + | |||
| + | ==October 7th== | ||
| + | |||
| + | ''Referencing the Notation used on October 6th.'' | ||
| + | |||
| + | *Minipreps: | ||
| + | -1a | ||
| + | -2a | ||
| + | -3a | ||
| + | -4a | ||
| + | -5a | ||
| + | -6a | ||
| + | |||
| + | *Lab Work | ||
| + | -Made Stock Solutions of the Minipreps | ||
| + | -Made SOB Media | ||
| + | -Made Competent Cells | ||
| + | -Poured Agar Plates | ||
| + | -Sent Samples to Sequencing Center | ||
| + | |||
| + | We began to anticipate the Jamboree at this point and were diligently working to finish on time. | ||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
| + | |||
| + | |||
| + | ==October 15 to 17== | ||
| + | Tecan data collected: | ||
| + | P6 promoter | ||
| + | |||
| + | D5 promoter | ||
| + | |||
| + | J23113 promoter | ||
| + | |||
| + | K091107 Lux promoter with J23108 expressing LuxR | ||
| + | |||
| + | K091107 Lux promoter with J23116 expressing LuxR | ||
| + | |||
| + | K091146 Lux promoter with J23108 expressing LuxR | ||
| + | |||
| + | K091146 Lux promoter with J23116 expressing LuxR | ||
Latest revision as of 03:59, 28 October 2010
Contents |
October 5th
- Digests:
-R1062+RFP (SP) -R0061+RFP (SP)
- Ligations:
-R1062+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
October 6th
We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.
- Colony PCR:
1. R1062+RFP + J23100+LuxR 2. R1062+RFP + J23108+LuxR 3. R1062+RFP + J23116+LuxR 4. R0063+RFP + J23100+LuxR 5. R0063+RFP + J23108+LuxR 6. R0063+RFP + J23116+LuxR
They were then run on a gel after being PCR'd.
- Gel Order:
- 1.a,b,c - 2.a,b,c - 3.a,b,c - 4.a,b,c - 5.a,b,c - 6.a,b,c
- Grown In Flasks:
-1a (R1062+RFP+J23100+LuxR) -2a (R1062+RFP+J23108+LuxR) -3a (R1062+RFP+J23116+LuxR) -4a (R0063+RFP+J23100+LuxR) -5a (R0063+RFP+J23108+LuxR) -6a (R0063+RFP+J23116+LuxR)
October 7th
Referencing the Notation used on October 6th.
- Minipreps:
-1a -2a -3a -4a -5a -6a
- Lab Work
-Made Stock Solutions of the Minipreps -Made SOB Media -Made Competent Cells -Poured Agar Plates -Sent Samples to Sequencing Center
We began to anticipate the Jamboree at this point and were diligently working to finish on time.
October 15 to 17
Tecan data collected: P6 promoter
D5 promoter
J23113 promoter
K091107 Lux promoter with J23108 expressing LuxR
K091107 Lux promoter with J23116 expressing LuxR
K091146 Lux promoter with J23108 expressing LuxR
K091146 Lux promoter with J23116 expressing LuxR
