From 2010.igem.org
(Difference between revisions)
|
|
| (One intermediate revision not shown) |
| Line 1: |
Line 1: |
| - | {{Team:Alberta/Head}}
| |
| | | | |
| - | {{Team:Alberta/navbar|project=selected}}
| |
| - |
| |
| - | {{Team:Alberta/beginLeftSideBar}}
| |
| - | {{Team:Alberta/endLeftSideBar}}
| |
| - |
| |
| - | {{Team:Alberta/beginRightSideBar}}
| |
| - | {{Team:Alberta/endRightSideBar}}
| |
| - |
| |
| - | {{Team:Alberta/beginMainContent}}
| |
| - |
| |
| - | Protocol 8: Ligation
| |
| - |
| |
| - |
| |
| - | Reagents:
| |
| - |
| |
| - | * Vector
| |
| - | * Insert
| |
| - | * MilliQ buffer
| |
| - | * 5X ligase buffer
| |
| - | * T4 DNA ligase
| |
| - | ** [http://tools.invitrogen.com/content/sfs/manuals/t4dnaligase_5U_man.pdf]
| |
| - |
| |
| - |
| |
| - | Procedure:
| |
| - |
| |
| - | *Volumes of vector and insert may vary, but a typical 20 ul reaction might be:
| |
| - | {|
| |
| - | | MilliQ water || 11 ul
| |
| - | |-
| |
| - | | 5x ligase buffer || 4 ul
| |
| - | |-
| |
| - | | Vector (200 ng/ul) || 1 ul
| |
| - | |-
| |
| - | | Insert (200 ng/ul) || 3 ul
| |
| - | |-
| |
| - | | Ligase || 1 ul
| |
| - | |}
| |
| - | *Incubate at R/T for 1 hours or overnight at 12-16<sup>o</sup>C.
| |
| - |
| |
| - |
| |
| - | [[Team:Alberta/Notebook/protocols| Back]]
| |
| - | {{Team:Alberta/endMainContent}}
| |
Latest revision as of 17:15, 26 October 2010
"
