Protocol/7

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Protocol 7: Gel Extraction
 
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Reagents:
 
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* Clean scalpel
 
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* Transilluminator
 
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* Eppendorf tube
 
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* Gel extraction kit
 
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* 55<sup>o</sup>C water bath
 
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* Vortex
 
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* Microfuge
 
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Procedure:
 
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* Place the gel on the transilluminator. Put on face shield and gloves.
 
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* Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
 
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* Pry out the gel ‘cube’ and place it in an eppendorf tube.
 
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* Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
 
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Latest revision as of 17:15, 26 October 2010