Week of 10/4

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==October 5th==
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 +
*Digests:
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-R1062+RFP (SP)
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-R0061+RFP (SP)
 +
 +
*Ligations:
 +
-R1062+RFP -->J23100+LuxR (B)
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            -->J23108+LuxR (B)
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            -->J23116+LuxR (B)
 +
-R0063+RFP -->J23100+LuxR (B)
 +
            -->J23108+LuxR (B)
 +
            -->J23116+LuxR (B)
 +
 +
==October 6th==
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 +
 +
We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.
 +
 +
 +
*Colony PCR:
 +
1. R1062+RFP + J23100+LuxR
 +
2. R1062+RFP + J23108+LuxR
 +
3. R1062+RFP + J23116+LuxR
 +
4. R0063+RFP + J23100+LuxR
 +
5. R0063+RFP + J23108+LuxR
 +
6. R0063+RFP + J23116+LuxR
 +
 +
They were then run on a gel after being PCR'd.
 +
 +
*Gel Order:
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- 1.a,b,c
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- 2.a,b,c
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- 3.a,b,c
 +
- 4.a,b,c
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- 5.a,b,c
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- 6.a,b,c
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 +
<center>[[Image:10-06-10.jpg]]</center>
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*Grown In Flasks:
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-1a (R1062+RFP+J23100+LuxR)
 +
-2a (R1062+RFP+J23108+LuxR)
 +
-3a (R1062+RFP+J23116+LuxR)
 +
-4a (R0063+RFP+J23100+LuxR)
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-5a (R0063+RFP+J23108+LuxR)
 +
-6a (R0063+RFP+J23116+LuxR)
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 +
==October 7th==
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''Referencing the Notation used on October 6th.''
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 +
*Minipreps:
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-1a
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-2a
 +
-3a
 +
-4a
 +
-5a
 +
-6a
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*Lab Work
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-Made Stock Solutions of the Minipreps
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-Made SOB Media
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-Made Competent Cells
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-Poured Agar Plates
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-Sent Samples to Sequencing Center
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 +
We began to anticipate the Jamboree at this point and were diligently working to finish on time.
 +
 +
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]
 +
 +
 +
==October 15 to 17==
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Tecan data collected:
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P6 promoter
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 +
D5 promoter
 +
 +
J23113 promoter
 +
 +
K091107 Lux promoter with J23108 expressing LuxR
 +
 +
K091107 Lux promoter with J23116 expressing LuxR
 +
 +
K091146 Lux promoter with J23108 expressing LuxR
 +
 +
K091146 Lux promoter with J23116 expressing LuxR

Latest revision as of 03:59, 28 October 2010

Contents

October 5th

  • Digests: 
-R1062+RFP (SP)
-R0061+RFP (SP)
  • Ligations: 
-R1062+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)

October 6th

We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.


  • Colony PCR: 
1. R1062+RFP + J23100+LuxR
2. R1062+RFP + J23108+LuxR
3. R1062+RFP + J23116+LuxR
4. R0063+RFP + J23100+LuxR
5. R0063+RFP + J23108+LuxR
6. R0063+RFP + J23116+LuxR

They were then run on a gel after being PCR'd.

  • Gel Order: 
- 1.a,b,c
- 2.a,b,c
- 3.a,b,c
- 4.a,b,c
- 5.a,b,c
- 6.a,b,c
10-06-10.jpg
  • Grown In Flasks: 
-1a (R1062+RFP+J23100+LuxR)
-2a (R1062+RFP+J23108+LuxR)
-3a (R1062+RFP+J23116+LuxR)
-4a (R0063+RFP+J23100+LuxR)
-5a (R0063+RFP+J23108+LuxR)
-6a (R0063+RFP+J23116+LuxR)

October 7th

Referencing the Notation used on October 6th.

  • Minipreps: 
-1a
-2a
-3a
-4a
-5a
-6a
  • Lab Work 
-Made Stock Solutions of the Minipreps
-Made SOB Media 
-Made Competent Cells
-Poured Agar Plates
-Sent Samples to Sequencing Center

We began to anticipate the Jamboree at this point and were diligently working to finish on time.


Return to Notebook


October 15 to 17

Tecan data collected: P6 promoter

D5 promoter

J23113 promoter

K091107 Lux promoter with J23108 expressing LuxR

K091107 Lux promoter with J23116 expressing LuxR

K091146 Lux promoter with J23108 expressing LuxR

K091146 Lux promoter with J23116 expressing LuxR

Retrieved from "http://2010.igem.org/Week_of_10/4"