Team:Stockholm/23 June 2010

From 2010.igem.org

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m (New page: {{Stockholm/Top2}} =Hassan= Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease. softwares used: #Molecular Maya #Chimera)
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{{Stockholm/Top2}}
{{Stockholm/Top2}}
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=Andreas=
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===Testing Top10 competent cells and antibiotic agar plates===
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''Results from 22/6''
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{| border="1" cellspacing="0" cellpadding="2"
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|'''Transformed plasmid'''
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|'''Plate'''
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|'''Growth'''
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|-
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|pEX
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|100 ug/ml Amp
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|Yes
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|-
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|None
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|100 ug/ml Amp
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|No
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|-
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|pEX
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|25 ug/ml Cm
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|No
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|-
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|None
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|25 ug/ml Cm
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|No
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|}
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Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.
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'' '''Note:''' The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.''
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===Plasmid preparation of pEX vector===
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Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.
 +
 +
===Transformation with pMA vector===
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The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).
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* Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.
=Hassan=
=Hassan=
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#Molecular Maya
#Molecular Maya
#Chimera
#Chimera
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{{Stockholm/Footer}}

Latest revision as of 10:31, 26 October 2010

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Contents

Andreas

Testing Top10 competent cells and antibiotic agar plates

Results from 22/6

Transformed plasmid Plate Growth
pEX 100 ug/ml Amp Yes
None 100 ug/ml Amp No
pEX 25 ug/ml Cm No
None 25 ug/ml Cm No

Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.

Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.

Plasmid preparation of pEX vector

Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.

Transformation with pMA vector

The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).

  • Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.

Hassan

Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.

softwares used:

  1. Molecular Maya
  2. Chimera





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/23_June_2010"