Team:Stockholm/25 October 2010

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|&#8627;&nbsp;&nbsp; [[Team:Stockholm/Lab_work/Safety|Safety]]
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|[[Team:Stockholm/Lab_work/Notebook|Notebook]]
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|[[Team:Stockholm/Lab_work/Protocols|Protocols]]
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{{Stockholm/Footer}}

Latest revision as of 15:04, 26 October 2010

Home Project Idea Lab Work Results Modelling Team       Follow us on and        2010.igem.org

↳   Notebook Safety Protocols



Mimmi

SOD activity

- continuing

mix samples blank 1 blank 3
sample solution 20
ddH2O 20 20
WST solution 200 200 200
Enzyme solution 20 20
dilution buffer 20
tot 240µl 240µl 240µl


  • Incubate in 37°C for 20 min


  • Measure A440 with nanodrop


  • SOD activity (inhibition %) = ((Ablank1 - Ablank3) - (Asample - Ablank2))/(Ablank1 - Ablank3) x 100


SOD activity2.png





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

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