Team:Stockholm/17 October 2010

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|&#8627;&nbsp;&nbsp; [[Team:Stockholm/Lab_work/Safety|Safety]]
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|[[Team:Stockholm/Lab_work/Notebook|Notebook]]
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|[[Team:Stockholm/Lab_work/Protocols|Protocols]]
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==== Gel ====
==== Gel ====
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[[Image:Place_for_picture.jpg|200px|thumb|left|]]
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[[Image:2010-10-17_pEX.SOD_operon.jpg|250px|thumb|left|]]
{|
{|
! well
! well
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| pC.Sh_yC 4
| pC.Sh_yC 4
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*pEX.SOD
*pEX.SOD
*pEX.yCCS
*pEX.yCCS
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{{Stockholm/Footer}}

Latest revision as of 14:52, 26 October 2010

Home Project Idea Lab Work Results Modelling Team       Follow us on and        2010.igem.org

↳   Notebook Safety Protocols




Contents

Mimmi

clone non-CPP operon

colony PCR

mix x12 primers samples
mastermix 24.5 pEX_VF pEX.SOD.RBS.yCCS 1-4
DNA 0.5 pEX_VR pEX.SOD.his.RBS.yCCS 1-4
tot 25µl pEX.his.SOD.RBS.yCCS 1-4
positive control (pSB1C3.LMWP.SOD.his)

Gel

2010-10-17 pEX.SOD operon.jpg
well sample well sample
1 ladder 12 ladder
2 pEX.S_yC 1 13 pEX.hS_yC 1
3 pEX.S_yC 2 14 pEX.hS_yC 2
4 pEX.S_yC 3 15 pEX.hS_yC 3
5 pEX.S_yC 4 16 pEX.hS_yC 4
6 pEX.Sh_yC 1
7 pC.Sh_yC 2
8 pC.Sh_yC 3
9 pC.Sh_yC 4



new colony PCR

mix x7 primers samples
mastermix 24.5 pEX_VF pEX.SOD.his.RBS.yCCS 1-7
DNA 0.5 pEX_VR
tot 25µl


ON cultures

  • pEX.SOD.RBS.yCCS 4
  • pEX.his.SOD.RBS.yCCS 3
  • pEX.SOD
  • pEX.yCCS





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/17_October_2010"