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Team:Stockholm/8 June 2010
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Contents |
Nina
Preparation of competent DH5α Escherichia coli cells
Continued from 21/6.
Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.
Absorbance:
- 1 ml sample from one bottle:
| Time | OD | |||
|---|---|---|---|---|
| 30min | 0,029 | |||
| 1h | 0,053 | |||
| 1h | 0,188 | |||
| 30min | 0,316 |
- 1 ml sample from two bottles:
| Time | OD | |||
|---|---|---|---|---|
| 30min | 0,519/0,503 | |||
| 7min | 0,578/0,566 | |||
| 5min | 0,598/0,600
|
Johan
Continuation of making competent cells
Step 2-12 in "800 ml of competent cells"
1 L instead of 800 ml -> 25 st 50 ml Falcon Tubes with 40 ml, 1 st with 15-20 ml
10) 16/23 = 0,69 ml
3) Abs:
Only one bottle
- 0,029 9:25
- 0,053 ~1h5min
- 0,188 ~1h5min
- 0,316 ~30min
Both bottles
- 0,519 ~30min
- 0,503
- 0,578 ~7min
- 0,566
- 0,598 ~5min = 13:05
- 0,600
12) ~160 epps, 100 µl in each.
They were then all put in the freezer, in two cardboard boxes (no metal blocks used)
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