Team:Northwestern/References
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+ | [[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]] | ||
- | == | + | <!--- The Mission, Experiments ---> |
- | + | {|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center" | |
+ | !align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]] | ||
+ | !align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]] | ||
+ | |} | ||
- | == | + | {| align="center" border="0" width="75%" |
+ | | | ||
+ | =='''References'''== | ||
- | + | === Lac Operon Control === | |
- | + | Lac Operon induction and IPTG: http://openwetware.org/wiki/IPTG | |
- | Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production | + | ===Chitin=== |
+ | |||
+ | Adding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886 | ||
+ | |||
+ | Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg] | ||
+ | |||
+ | Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long | ||
+ | |||
+ | Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html | ||
=== Chitin Synthesis === | === Chitin Synthesis === | ||
- | + | Chitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311 | |
- | CHS3 | + | CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465 |
===Chitin Staining (Calcofluor)=== | ===Chitin Staining (Calcofluor)=== | ||
- | + | Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf | |
- | + | Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za | |
- | + | Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/ | |
- | + | Data sheet: http://tinyurl.com/283wax2 | |
- | + | Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html | |
+ | === Apoptosis === | ||
- | + | partsregistry: http://partsregistry.org/Cell_death | |
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=== Live/Dead Staining aka Viability/Cytotoxicity Assay=== | === Live/Dead Staining aka Viability/Cytotoxicity Assay=== | ||
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*http://www.biotium.com/product/product_info/Protocol/30027.pdf | *http://www.biotium.com/product/product_info/Protocol/30027.pdf | ||
- | ==Keio Collection - KnockOuts== | + | ===Keio Collection - KnockOuts=== |
- | Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html | + | Keio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html |
Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp | Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp | ||
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Method: http://www.pnas.org/content/97/12/6640.full.pdf+html | Method: http://www.pnas.org/content/97/12/6640.full.pdf+html | ||
- | Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html | + | Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html |
Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls | Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls | ||
+ | === ChiA (Chitinase) Knockout === | ||
+ | Summary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | ||
- | + | Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837 | |
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chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | ||
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===ydgg/tqsA (AI2-Transporter) Knockout=== | ===ydgg/tqsA (AI2-Transporter) Knockout=== | ||
- | + | Knockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 | |
- | + | ||
- | http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 | + | |
more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf | more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf | ||
- | == BIF (Biological Imaging Facility)== | + | === BIF (Biological Imaging Facility)=== |
List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br /> | List of Equipment: http://www.northwestern.edu/bioimaging/equip.html <br /> | ||
Methods: http://www.northwestern.edu/bioimaging/methods.html | Methods: http://www.northwestern.edu/bioimaging/methods.html | ||
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+ | |} |
Latest revision as of 01:18, 27 October 2010
Home | Brainstorm | Team | Acknowledgements | Project | Human Practices | Parts | Notebook | Calendar | Protocol | Safety | Links | References | Media | Contact |
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ReferencesLac Operon ControlLac Operon induction and IPTG: http://openwetware.org/wiki/IPTG ChitinAdding Glucosamine in medium: http://ec.asm.org/cgi/reprint/2/5/886 Chitin synthesis Pathway (image): [http://www.mekarn.org/msc2005-07/thesis07/latslrimage002.jpg] Chitin Precursor (UDP-N-Acetyl-Glucosamine) is used for Endotoxin (Lipid A) production: http://www.jbc.org/content/268/26/19858.long Uses for Chitin: http://www.gmp-chitosan.com/en/products-services/chitin.html Chitin SynthesisChitin Synthase Gene (CHS3): http://www.ncbi.nlm.nih.gov/gene/852311 CHS3 polymerizes: http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004835, http://www.uniprot.org/uniprot/P29465 Chitin Staining (Calcofluor)Effect on e. coli: http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf Staining of Biofilm Polysaccharides- http://tinyurl.com/36pn4za Inhibition of Chitin Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/ Data sheet: http://tinyurl.com/283wax2 Excitation/Emission: http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html Apoptosispartsregistry: http://partsregistry.org/Cell_death Live/Dead Staining aka Viability/Cytotoxicity AssayDMAO(Live), EtD-III(Dead)
Keio Collection - KnockOutsKeio Website: http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html Ordering: http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp Method: http://www.pnas.org/content/97/12/6640.full.pdf+html Journal Paper: http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html Primer Extensions: http://www.biomedsearch.com/attachments/00/16/73/85/16738554/msb4100050-s4.xls ChiA (Chitinase) KnockoutSummary of e. coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts Chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837 chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 ydgg/tqsA (AI2-Transporter) KnockoutKnockout biofilm formation: http://jb.asm.org/cgi/content/short/188/2/587, http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593 more info: http://www.open-access-biology.com/biofilms/biofilmsch7.pdf BIF (Biological Imaging Facility)List of Equipment: http://www.northwestern.edu/bioimaging/equip.html
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