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Team:Panama/29 July 2010
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==='''July 29'''=== | ==='''July 29'''=== | ||
| - | We performed the E. coli transformation again, with three BioBrick parts. In order to check the success of the extraction, we made an electrophresis in a 1% agarose gel. | + | We performed the ''E. coli'' transformation again, with three BioBrick parts. In order to check the success of the extraction, we made an electrophresis in a 1% agarose gel. |
''Electrophoresis'' | ''Electrophoresis'' | ||
| - | 70mL of TBE 1X | + | 0,7g of agarose weew dissolve 70mL of TBE 1X. |
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In each well, we added 2ul of loading buffer and 5ul of the sample. Final volume 7ul. | In each well, we added 2ul of loading buffer and 5ul of the sample. Final volume 7ul. | ||
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| + | [[Image:GEL29JULIO.JPG|250px|thumb|left|alt text]] | ||
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| + | [[Image:DSC02595.JPG|250px|thumb|left|alt text]] | ||
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| + | The bands that were obtained corresponded to the expected results and this proves the success of the plasmid DNA miniprep. | ||
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} | ||
Latest revision as of 04:04, 27 October 2010
July 29
We performed the E. coli transformation again, with three BioBrick parts. In order to check the success of the extraction, we made an electrophresis in a 1% agarose gel.
Electrophoresis
0,7g of agarose weew dissolve 70mL of TBE 1X.
In each well, we added 2ul of loading buffer and 5ul of the sample. Final volume 7ul.
Agarose gel wells:
The bands that were obtained corresponded to the expected results and this proves the success of the plasmid DNA miniprep.
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