Team:Stockholm/21 June 2010
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== Preparation LB agar plates with antibiotics == | == Preparation LB agar plates with antibiotics == | ||
- | * 100 ug/ml Amp | + | * 100 ug/ml Amp (20 plates) |
- | * 25 ug/ml Cm | + | * 25 ug/ml Cm (20 plates) |
+ | |||
+ | == Preparation of competent Top10 Escherichia coli cells == | ||
+ | * Set a 5 ml LB ON culture; 37°C, 250 rpm. | ||
+ | |||
+ | =Johan= | ||
+ | |||
+ | Overnight culture of pEX | ||
+ | |||
+ | 3 falcon tubes (50 ml), 10 ml LB in each. | ||
+ | |||
+ | 24/12=2 | ||
+ | |||
+ | 2*30=60µl Amp | ||
+ | |||
+ | with tip from pEX glycerol stock | ||
+ | |||
+ | {{Stockholm/Footer}} |
Latest revision as of 18:04, 27 October 2010
Contents |
Andreas
Preparation LB agar plates with antibiotics
- 100 ug/ml Amp (20 plates)
- 25 ug/ml Cm (20 plates)
Preparation of competent Top10 Escherichia coli cells
- Set a 5 ml LB ON culture; 37°C, 250 rpm.
Johan
Overnight culture of pEX
3 falcon tubes (50 ml), 10 ml LB in each.
24/12=2
2*30=60µl Amp
with tip from pEX glycerol stock