Team:Northwestern/Notebook

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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
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__NOTOC__
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<!--- The Mission, Experiments --->
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{|- style="background:#FFFFFF; style="font-size: 87%;" color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"
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!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
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!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]
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!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
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!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]
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!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
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!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
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!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
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!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
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!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
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!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]
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!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]
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!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]
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!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]
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===6/14-18/10 (Boot Camp)===
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/* Wiki Hacks - END */
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DNA extracted from kit
 
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Part: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I746200]
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}
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===6/22/10===
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#menubar li a {
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We poured our Kan, Amp, Tet, Kan+Amp, Kan+Tet, Amp+Tet antibiotic agar plates.
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<u>Antibiotic Concentrations</u>
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*Amp: 50mg/500ml
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}
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*Kan: 31.97mg/500ml
 
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*Tet: 6mg/500ml
 
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----
 
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===6/23/10===
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#footer-box {
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Resuspended 12O and 6G DNA from the iGEM Kit and transformed them into our competent cells.
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6G = r0010(inducible promoter)
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#footer-box a {
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12O = e0840(rbs30-gfp-2xterm)
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----
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/* Wiki Hacks - END */
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</style>
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</html>
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===6/24/10===
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<html>
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Ran a gel of our PCR product to make sure that our taq polymerase master mix was working.  
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<head>
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<style>
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  background: #EFCDF8; <!-- EEDCC8 url(https://static.igem.org/mediawiki/2010/3/38/BG1.jpg) repeat-y; -->
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</html>
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Selected single 12O and 6G colonies and transferred them to LB and incubated for 18 hours.
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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
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Let 1 12O and 1 6G plate incubate at RT. Let 1 12O and 1 6G plate incubate at 37°C. Intended to observe lawn growth.
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<!--- The Mission, Experiments --->
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{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
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!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
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!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
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!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
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!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
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!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
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!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
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!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
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!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
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!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
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!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
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!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
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!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
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!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
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|}
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----
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{| align="center" border="0" width="75%"
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|
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='''Notebook'''=
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===6/25/10===
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[[Brainstorming April-June 2010]]
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Low DNA yield; Plan to redo DNA extraction from Kit
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Found red/pink colonies in 12O plates. Thought to be contaminations. Plates were thrown out.
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NOTE: All work done by the undergraduate students of NU iGEM.
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=='''June'''==
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[[Week1 6/13/10-6/19/10]]
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----
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[[Week2 6/20/10-6/26/10]]
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===6/28/10===
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[[Week3 6/27/10-7/3/10]]
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Kit to Stock
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'''1-12O''' (r0010: inducible promoter)
 
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'''1-6G''' (e0840: rbs30-gfp-2xterm)
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=='''July'''==
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[[Week4 7/4/10-7/10/10]]
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'''3-20M''' (K124017: Bacteriophage Lysis Cassette S105, R, and Rz)
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[[Week5 7/11/10-7/17/10]]
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Plates were incubated at 37°C overnight (starting at 2:30 pm).
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[[Week6 7/18/10-7/24/10]]
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----
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[[Week7 7/25/10-7/31/10]]
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===6/29/10===
 
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Selected single 1-12O, 1-6G, 3-20M colonies and grew them in Amp-LB broth for 18 hours (starting at 4:15 pm).
 
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===6/30/10===
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=='''August'''==
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[[Week8 8/1/10-8/7/10]]
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Poured 2 Sleeves of Kan and Amp plates respectively
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[[Week9 8/8/10-8/14/10]]
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Met with Dr. Russin (BIF)
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[[Week10 8/15/10-8/21/10]]
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Sent emails regarding biofilm and biofilm imaging: Aaron Packman, Wei Zhang, Kimberly Grey
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[[Week11 8/22/10-8/28/10]]
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Most likely going to use water immersion Leica (Confocal Microscope) + low MP agar (enrobing technique)
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[[Week12 8/29/10-9/4/10]]
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Red colonies reappeared on 1-12O plates; Fluorescent Microscopy revealed negligible activity; still unsure why colonies are red.
 
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Miniprepped 1-12O, 3-20M, 1-6G: nanodrop -> mostly below 20ng, 1 above 100ng; stored in -20°C
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=='''September'''==
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[[Week13 9/5/10-9/11/10]]
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Re-miniprepped after growing in liquid LB for 3 hours; nanodrop -> around 50ng, 2 around 100ng; stored in -20°C
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[[Week14 9/12/10-9/18/10]]
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Transferred overnight cultures into new 5ml LB broths. Incubated overnight in an angled shaker (starting from 4:20pm)
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[[Week15 9/19/10-9/25/10]]
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Plated 10μl, 25μl, 100μl, and 200μl (added LB up to 200μL) of the KR 1-12O overnight culture. Intend to measure the thickness of E.Coli lawn.
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[[Week16 9/26/10-10/2/10]]
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===7/1/10===
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=='''October'''==
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[[Week17 10/3/10-10/9/10]]
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First weekly meeting with Professor Jewett, Leonard, and Mordacq.
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Removed overnight cultures from incubator at 10:00am. Miniprep (let the elution buffer sit for 10 minutes with the DNA before centrifuging)  --> 1 around 30ng/μL, mostly around 75ng/μL.
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[[Week18 10/10/10-10/16/10]]
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Kit to Stock
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[[Week19 10/17/10-10/23/10]]
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===7/2/10===
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[[Week20 10/24/10-10/30/10]]
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----
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=='''November'''==
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[[Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives]]
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|}

Latest revision as of 05:54, 20 March 2011


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Notebook

Brainstorming April-June 2010

NOTE: All work done by the undergraduate students of NU iGEM.

June

Week1 6/13/10-6/19/10

Week2 6/20/10-6/26/10

Week3 6/27/10-7/3/10


July

Week4 7/4/10-7/10/10

Week5 7/11/10-7/17/10

Week6 7/18/10-7/24/10

Week7 7/25/10-7/31/10


August

Week8 8/1/10-8/7/10

Week9 8/8/10-8/14/10

Week10 8/15/10-8/21/10

Week11 8/22/10-8/28/10

Week12 8/29/10-9/4/10


September

Week13 9/5/10-9/11/10

Week14 9/12/10-9/18/10

Week15 9/19/10-9/25/10

Week16 9/26/10-10/2/10


October

Week17 10/3/10-10/9/10

Week18 10/10/10-10/16/10

Week19 10/17/10-10/23/10

Week20 10/24/10-10/30/10


November

Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives

Retrieved from "http://2010.igem.org/Team:Northwestern/Notebook"