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Team:Stockholm/21 June 2010
From 2010.igem.org
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(New page: {{Stockholm/Top2}} = Andreas = == Preparation LB agar antibiotic plates == * 100 ug/ml Amp * 25 ug/ml Cm) |
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| - | == Preparation LB agar | + | == Preparation LB agar plates with antibiotics == |
| - | * 100 ug/ml Amp | + | * 100 ug/ml Amp (20 plates) |
| - | * 25 ug/ml Cm | + | * 25 ug/ml Cm (20 plates) |
| + | |||
| + | == Preparation of competent Top10 Escherichia coli cells == | ||
| + | * Set a 5 ml LB ON culture; 37°C, 250 rpm. | ||
| + | |||
| + | =Johan= | ||
| + | |||
| + | Overnight culture of pEX | ||
| + | |||
| + | 3 falcon tubes (50 ml), 10 ml LB in each. | ||
| + | |||
| + | 24/12=2 | ||
| + | |||
| + | 2*30=60µl Amp | ||
| + | |||
| + | with tip from pEX glycerol stock | ||
| + | |||
| + | {{Stockholm/Footer}} | ||
Latest revision as of 18:04, 27 October 2010
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Contents |
Andreas
Preparation LB agar plates with antibiotics
- 100 ug/ml Amp (20 plates)
- 25 ug/ml Cm (20 plates)
Preparation of competent Top10 Escherichia coli cells
- Set a 5 ml LB ON culture; 37°C, 250 rpm.
Johan
Overnight culture of pEX
3 falcon tubes (50 ml), 10 ml LB in each.
24/12=2
2*30=60µl Amp
with tip from pEX glycerol stock
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