Team:WashU

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{{WashUHeader}}
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==Introduction / Abstract==
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''Saccharomyces cerevisiae'' is a model unicellular eukaryotic chassis; however when compared with Escherichia coli the available synthetic biology tools are lacking. To remedy this problem the 2010 Washington University iGEM team has introduced a synthetic alternative splicing tool, as well as designed and produced new BioBricks parts to ease transformation of synthetic constructs into ''S. cerevisiae''. A mutually exclusive exon splicing system was formulated in which Sex-lethal interacts with the native splicing machinery to affect splice site choice. Two vectors have been designed to facilitate simple bacterial BioBrick manipulation and subsequent chromosomal integration into the yeast genome. A yeast positive selection marker BioBrick has been produced for the first time. Chromosomal integration with positive selection will stabilize and streamline BioBrick transformations into ''S. cerevisiae''. A synthetic splicing assembly will allow for new synthetic biology techniques such as isoform engineering of proteins or combinatorial logic.
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==Sponsors==
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<a href="http://www.sigmaaldrich.com/sigma-aldrich/home.html"><img src="https://static.igem.org/mediawiki/2010/8/80/Sigma.png"></a></center></html>
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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==Acknowledgements==
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*Sigma Aldrich has generously donated the reagents used during the course of our experiment.
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*We would like to thank Dean Quatrano and the Washington University in St. Louis Department of Biomedical Engineering for there assistance and funds throughout the entire summer.
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*We would also like to thank the Washington University in St. Louis Department of Biology and Carol Kohring for their lab space, equipment and assistance in the Lab.
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*The Summer Undergraduate Research Fellowship (SURF), Washington University Career Center, Mckelvey  Scholarship Program and the Tang Lab for providing stipends.
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*The assistance of the Cohen Lab, Dantas Land, Jez Lab and Yinjie Lab.
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==Contact Us==
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If you have any questions, advice, or are interested in joining the 2011 WashU iGEM team we would love to hear from you.  The Washington University iGEM team may be reached at [mailto:WashU.iGEM@gmail.com WashU.iGEM@gmail.com]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:WashU | Team Example]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:WashU|Home]]
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!align="center"|[[Team:WashU/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=WashU Official Team Profile]
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!align="center"|[[Team:WashU/Project|Project]]
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!align="center"|[[Team:WashU/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:WashU/Modeling|Modeling]]
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!align="center"|[[Team:WashU/Notebook|Notebook]]
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!align="center"|[[Team:WashU/Safety|Safety]]
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Latest revision as of 00:07, 9 April 2011


Introduction / Abstract

Saccharomyces cerevisiae is a model unicellular eukaryotic chassis; however when compared with Escherichia coli the available synthetic biology tools are lacking. To remedy this problem the 2010 Washington University iGEM team has introduced a synthetic alternative splicing tool, as well as designed and produced new BioBricks parts to ease transformation of synthetic constructs into S. cerevisiae. A mutually exclusive exon splicing system was formulated in which Sex-lethal interacts with the native splicing machinery to affect splice site choice. Two vectors have been designed to facilitate simple bacterial BioBrick manipulation and subsequent chromosomal integration into the yeast genome. A yeast positive selection marker BioBrick has been produced for the first time. Chromosomal integration with positive selection will stabilize and streamline BioBrick transformations into S. cerevisiae. A synthetic splicing assembly will allow for new synthetic biology techniques such as isoform engineering of proteins or combinatorial logic.

Sponsors

Acknowledgements

  • Sigma Aldrich has generously donated the reagents used during the course of our experiment.
  • We would like to thank Dean Quatrano and the Washington University in St. Louis Department of Biomedical Engineering for there assistance and funds throughout the entire summer.
  • We would also like to thank the Washington University in St. Louis Department of Biology and Carol Kohring for their lab space, equipment and assistance in the Lab.
  • The Summer Undergraduate Research Fellowship (SURF), Washington University Career Center, Mckelvey Scholarship Program and the Tang Lab for providing stipends.
  • The assistance of the Cohen Lab, Dantas Land, Jez Lab and Yinjie Lab.


Contact Us

If you have any questions, advice, or are interested in joining the 2011 WashU iGEM team we would love to hear from you. The Washington University iGEM team may be reached at WashU.iGEM@gmail.com

Retrieved from "http://2010.igem.org/Team:WashU"