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User:MayaBrandi/1 July 2010
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Mayabrandi (Talk | contribs) (Preparations for PPI study of the differentially expressed proteins in melanocytes) |
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| - | 1 | + | 1 Microarrays - reliability |
| - | + | - How reliable is the protein regulation profile in the article "Transcriptional profiling of melanocytes..." | |
2 How should one priorate different types of protein-protein interactions? | 2 How should one priorate different types of protein-protein interactions? | ||
| - | + | Focus on experimental (not predictive ) data and focus first of all at physical PPI second at co expression and co localisation. Skip the rest. | |
| - | + | To improve PPI reliability | |
| - | + | * Find a good coverage from many primary databases. -How many and with? | |
| - | + | * What experiments were used? If the same result is dereved from many different experiments then the PPI is more reliable. | |
| - | + | * Look at 3D structures...? | |
| - | + | Compare with canonical pathways to get a better insight in the functional roles of the proteins in the network. | |
Latest revision as of 07:41, 2 July 2010
1 Microarrays - reliability - How reliable is the protein regulation profile in the article "Transcriptional profiling of melanocytes..."
2 How should one priorate different types of protein-protein interactions? Focus on experimental (not predictive ) data and focus first of all at physical PPI second at co expression and co localisation. Skip the rest.
To improve PPI reliability
- Find a good coverage from many primary databases. -How many and with?
- What experiments were used? If the same result is dereved from many different experiments then the PPI is more reliable.
- Look at 3D structures...?
Compare with canonical pathways to get a better insight in the functional roles of the proteins in the network.
