Team:LMU-Munich/Jump-or-die/Functional Principle
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== Jump-or-die-System == | == Jump-or-die-System == | ||
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The Jump-or-Die-system facilitates the integration of a gene of interest into cellular genome and selects the cells successfully integrated with the gene of interest by apoptosis. Therefore three constructs are needed. | The Jump-or-Die-system facilitates the integration of a gene of interest into cellular genome and selects the cells successfully integrated with the gene of interest by apoptosis. Therefore three constructs are needed. | ||
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== Transfection possibilities == | == Transfection possibilities == | ||
- | A cellline which has construct 1 integrated into the genome will be co-transfected with construct 2 and 3. Construct 3 will be read-off instantly, in contrast to construct 1 which has to be induced by | + | A cellline which has construct 1 integrated into the genome will be co-transfected with construct 2 and 3. Construct 3 will be read-off instantly, in contrast to construct 1 which has to be induced by doxycycline and because of the lack of a promoter, construct 2 will at the beginning not be translated. |
The outgoing of this co-transfection has the following possibilities: | The outgoing of this co-transfection has the following possibilities: | ||
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[[Image: Dnajump7.PNG | 600px | Jump recombination]] | [[Image: Dnajump7.PNG | 600px | Jump recombination]] | ||
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Latest revision as of 14:45, 25 October 2010